Gene therapy : the Wellcome Trust lecture 1993 / Marcus Pembrey.

  • Marcus Pembrey
Date:
1993
Catalogue details

Licence: Attribution 4.0 International (CC BY 4.0)

Credit: Gene therapy : the Wellcome Trust lecture 1993 / Marcus Pembrey. Source: Wellcome Collection.

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    The simplest way to add new DNA to the cells is to copy what the sperm does and 'get in at the beginning'. Genetic modification of laboratory animals (often to create animal models of human genetic disease in order to devise new treatments) is usually done by adding the DNA at the beginning of development. This would lead to genetic modification of the germline cells and is outlawed in humans. Methods for gene delivery to cells may be physical or viral. Gaining entry - Physical ♦ INJECTION ♦ ZAPPING (electroporation) A brief electric shock causes transient holes in the cell membrane to let the DNA in ♦ MEMBRANE MERGE (liposome-mediated-gene-transfer) The new DNA is placed in artificial / natural lipid - bilayer vesicles that will fuse with the cell membrane ♦ THE IMPOSTER (receptor - mediated - endocytosis) The new DNA is attached to a protein that normally enters cells via a special receptor
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