Proteins : lectures given in the United States of America in 1924 / by S.P.L. Sørensen.

  • Sørensen, S. P. L. (Soren Peter Lauritz), 1868-1939.
Date:
1925
Catalogue details

Licence: In copyright

Credit: Proteins : lectures given in the United States of America in 1924 / by S.P.L. Sørensen. Source: Wellcome Collection.

    38/174 (page 12)
    The solubility of this well-washed sample of euglobulin is thus very nearly proportional to the amount of globulin em- ployed in the solubility experiment, just as stated by Mellanby and Hardy. A really effective purification cannot, however, be obtained by washing with water, but only by dissolving the euglobulin in a weak salt solution and subsequent precipitation by dilution with water. To throw light on this question I shall mention a Diagram 5 Purification of the Globulin Fraction Bin Nature of the Globulin Fraction Bin Biv [Bin dissolved in 125 cc. of H2O + 15 cc. of N NaCl, precipitated with 1 liter of H2O; decantation of the mother liquor, washing with 250 cc. of H2O, decanta- tion of the washing water and suspen- sion of Biv in 200 cc. of H2O]. Bv [Biv dissolved in 140 cc. of H2O + SO cc. of N NaCl, precipitated with 1 liter of H2O; decantation of the mother liquor, washing with 200 cc. of H2O, decanta- tion of the washing water and suspen- sion of Bv in 95 cc. of H2O]. Bvi [Bv dissolved in 45 cc. of H2O + 35 cc. of N NaCl, precipitated with 850 ec. of H2O; decantation of the mother liquor, wash- ing with 190 cc. of H2O, decantation of the washing water and suspension of Bvi in 100 cc. of H2O]. Solubility of the globulin fraction in 0.02 N NaCl solution Total amount of globulin nitrogen per 100 cc. used for the ex- periment mg. Amount of globulin nitrogen dis- solved per 100 cc. of solution mg. Mg. of P per g. of globulin nitrogen 26.22 52.44 78.66 3.23 5.39 6.89 • 4 11.92 23.84 1.13 1 1.64 ► 4 35.76 2.05 20.71 0.82 ' 41.42 0.87 ► 6 62.13 1.28 17.08 0.62 ‘ 34.16 0.62 10 51.24 0.72 ,