A note on hæmolysin and hæmagglutinin with reference to the Wassermann Reaction / C.C. Okell and H.J. Parish.

  • Okell, C. C. (Charles Cyril), 1888-1939.
Date:
[1924?]
Catalogue details

Licence: In copyright

Credit: A note on hæmolysin and hæmagglutinin with reference to the Wassermann Reaction / C.C. Okell and H.J. Parish. Source: Wellcome Collection.

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    (1) the production of haemolysin (Bordet, 1898; Ehrlich and Morgenroth, 1899), (2) the production of haemagglutinin, and (3) the gradually increasing tolerance of the animal to the injections, i.e. the development of immunity in the true sense. This is indicated, though only roughly, by the dosage of red cells recorded on the chart, since the reaction produced by each dose suggested that it was practically the maximum which could be tolerated by the animal at the time of injection without serious symptoms. i CHART 2 CHART I Ordinates <ar<2. proporKonal fo tea. log. of Hie reciprocals of fha. dilufions of aerom necessary fo produce complete haemolysis or haemayaluhnahon. 1 c.c of I ir\ 20 complement- and I c.c. of 2'5/o Red Cells are added to com- pl&te the haemolytic system. CHART 3 V/eaks I in 1000 Waeks Weeks I in 10000 I in 1000 Waak.3 In our experience each progressive course of injections of red cells is followed by all three of these phenomena. It will be seen from the chart that, following the first small injection, there is a latent period of four to eight days, then a rapid rise of haemolysin which may reach a titre of 1 in 1000 or more with hardly detectable agglutinin formation. A little later the agglutinins also begin to rise in titre, at first slowly, then much more rapidly. Once the agglutinins begin to show rapid development the rate of increase of hsemolysin production tends to become relatively slow. Soon the haemolytic titre shows no further rise in response to injections, and indeed it may begin to fall. Chart 3 illustrates practically the same phenomena, but as a result of delay in making a full bleeding of the animal, the falling curve of the haemolytic
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