Gout : its pathology and treatment / by Arthur P. Luff.

  • Arthur P. Luff
Date:
1898
Catalogue details

Licence: Public Domain Mark

Credit: Gout : its pathology and treatment / by Arthur P. Luff. Source: Wellcome Collection.

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    readiness. Another great advantage of the pro- cess is that although xanthin is at first precipitated along with the ammonium urate, yet the subse- quent treatment with hydrochloric acid entirely removes it, so that finally it is not estimated along with the uric acid. THE GOWLAND-HOPKENS METHOD FOR THE ESTIMATION OF URIC ACID IN URINE. To 100 c.c. of the urine powdered ammonium chloride is added till practical saturation is ob- tained ; about 30 grammes of ammonium chloride as a rule are required. When a small quantity remains undissolved, after brisk stirring for a few minutes, saturation is sufficiently complete. The urine is then allowed to stand for two hours, during which time, if possible, it is occasion- ally stirred to promote subsidence, and is then filtered through thin filter-paper, and washed three or four times with a saturated solution of ammonium chloride. The filtrate should remain perfectly clear and bright. The precipitated ammonium urate is then washed off' the filter into a small beaker with a jet of hot distilled water, and is heated just to boiling with an excess of hydro- chloric acid. The beaker and its contents are allowed to stand in the cold for two hours, when the uric acid separates out completely, and is then collected on a filter and washed with cold distilled water. The filtrate should be measured before the washing is begun, and one milligramme added to the final result for each 15 c.c. of filtrate present—