The immune system: genes, receptors, signals : [proceedings] / edited by Eli E. Sercarz, Alan R. Williamson [and] C. Fred Fox.

  • ICN-UCLA Symposium on Molecular Biology
Date:
1974
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Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: The immune system: genes, receptors, signals : [proceedings] / edited by Eli E. Sercarz, Alan R. Williamson [and] C. Fred Fox. Source: Wellcome Collection.

    653/664 page 625
    THE IMMUNE SYSTEM Experiments with combinations differing in the and ^ regions, [A.TL versus A.TH; B10.T(6R) versus BIO.AQR]: In these combinations, T and В cells stimulated to an equal extent or in some cases (A.TL versus A.TH) T cells stimula¬ ted slightly better than В cells, (Table 9, experiments 1 and 2, and Table 10). Combinations differing in the Ir-lA and Ir-lB subregions [(A,TLxB10/Sn)Fi versus BlO.HTT]: In this combination В cells gave a significantly weaker stimulation than T cells (Table 9, experiment 3). Combinations differing in a region between Ir-lB and Sip (subregion [42].) [ (B10.HTTxA/J)Fi versus A.TH; and B10.S(7R) versus BlO.HTT]: In these combinations, only T cells stimulated; with В cells as stimulator cells, no sig¬ nificant degree of stimulation could be obtained (Table 9, experiments 4 and 5). These findings suggest that antigens controlled by the region are expressed on both T and В cells, as indicated by the MLR reaction. The fact that strain combinations exist which differ primarily in T-cell antigens, implies that at least some of the genes controlling the stimulatory structures are expressed exclusively on given lymphocyte sub- populations., A similar observation has been made by Fathman et al. [43], who found stimulation exclusively by В cells in the B10.A(4R) versus B10.A(2R) and the BIO.A versus B10.D2 combination. The genetic difference between these combina¬ tions, however, comprises a larger chromosome segment than that tested in our last combination above. We have compared our data with the genetic localization of la antigens, as described by Shreffler's group [23,42], and found good agreement. Specifically, the combinations differing in specificities assigned to the third I subregion (I-C) separ¬ ate this subregion and describe only T cell antigens as recognized in the MLR. The MLR combinations also correspond well with the la specificities assigned to the subregions according to their H-2 genotype. If these correlations can be continued in following experiments, then there will be a good possibility of investigating the specificity of the MLR and the possible identity of the Lad and la loci. To inves¬ tigate the relationship between Lad loci and I^ genes, other studies will be needed. The comparison of the map position of Ir genes and Lad loci and their cellular distribution migívt give useful information provided that narrow enough genetic regions can be studied by the use of appropriate 625
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