Some observations on the antiseptic and physiological action of resorcin / by W.B. Platt.
- Platt, Walter Brewster, 1853-1922.
- Date:
- 1883
Licence: Public Domain Mark
Credit: Some observations on the antiseptic and physiological action of resorcin / by W.B. Platt. Source: Wellcome Collection.
Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.
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![Skkiks a—Experiwent conccrniwj Action of Resorcin upon Serum of Ox-Blood. hxpt. —March G, iit. 4.40 I\ M. 'I’lie scrmn from ox-l)Ion(] obtaiiiod from tlie amimi tins monun^r was filtorc-.l tliroufrl, animal clmiroal, afu-r wliicli it ap])cars pale, red, almo.st transparent, sliowinn nndcr the mieroseope no bacteria only a.few red b ood-corpnseles. Of this, 20 e c. lluidraclim.s) were plaee.l in a clean wineplass just washed with boilinji distilled water. 1 e. c. of a .0 iier cent, .sol (aipieons) of re.sorcin (= O.OoO gram. = f grain) was added. The glass with Its contents was next jilaced in an incnbating closet, covered, and kept at a temperature of 3S°~:irfi Centigrade. This was labelled “ 1.” hxpt. 1. 11 c. c. (about .3 drachms) of serum in a wineglass without addition of resorcin was subjected to the same conditions otherwise, jiluced in the incu- bator along with the resorcin-serum, and labelled “ 1*.” hxpt. \. Serum with resorcin.—il/«rcA 7, 3.45 P. :M. After 23 hours. J cnip. 35'^ C. Clear, pale red. A few white flakes adherent to sides of glass. A very slight sediment. Reaction faintly alkaline; odour fragrant. Rarely bacteria are seen, d’hose are not in motion. ^ 8t/i, 12.40 P. INI. After 44 hours. _ Icinj). 35® C. Fluid dark red or smoky ; otherwise same as last. A few motionless bacteria present. 9//i, 3.45 P. ]\1. After 71 hours. 'J'emp. 35® C. Upper layer of scrum dark oHvivgrcen; fluid opaipie. Reaction alkaline. I^loriiKj organisms present. hxpt. 1*. Serum without resorcin. —.4/orc// 7, 3.45 P. M. After 23 hours, 'lemp. 35® C. Turbid ; redder than Xo. 1 ; otherwise the same, excepting that numerous rod-shaped, moving bacteria nrn present. Conclusion.— ^ (.0.50) grain of re.sorcin retards the active development of bacteria in 5 drachms (20 c. c.) of ox-blood serum at least 21 hours. 8ERIKS B.— Concerning xintiseptic Action of Resorcin and Carbolic Acid upon Normal Urine originally free from Bacteria. hxpt. 2.—March G, 1882, 5.15 P. M. Recently passed normal urine, faintly acid, transj)arent, and having as a sediment only an occasional epithelial scale or minute foreign body, is employeil. Entire absence of bacteria large enough to be seen with a power of 220 diam. (Zeiss obj. D, ocular No. 2). in each of three wineglasses, labelled 2‘, 2^, 2®, is placed 20 c.c. of urihe. To each glass 1 c. c. of the resorcin 5 per cent. sol. is added. They are placed in the hot closet and maintained at 30®-35® C., mostly 34® C. (each glass covered with a ground- glass plate). Two glasses labelled 2“ and 2*> containing each 20 c. c. of urine, without addition of resorcin, subjected to the same conditions, and likewise placed in hot closet. 7t/i, 11.20 A. M. After 18 hours, two of the glasses containing urine with resorcin, viz., 2^ and 2®, contain a few bacteria in motion. Urine appears other- wise normal. Urine alone, 2“ and 2*', without re.sorcin, is strongly alkaline, pale, has an iridescent pellicle, contains crystals of triple phosphate, and many moving bacteria. \Uh. After G days,* 2® has become opaque, very black (almost like ink), strongly alkaline. Conclusion.—0.050 (= | grain) of resorcin has a very slight influence in retarding development of bacteria in 20 c. c. of urine, for a time, within 18 hours. Expt^'s.—March 7, 1882, 4.30 P. M. Clear, yellow, freshlv passed, slightly acid urine, free from bactei ia, was employed. 20 c. c. of this is placed in each of three wiiu'frlasses which contain 2 c. c. each of a 5 per cent, acjueous solution of reson in. These are labelled respectively 3', 3*, and 3®. 20 c. c. ot same, urine without resorcin placed in a marked gla.ss, and about 40 c. c. of urine ]>laced in a llask stopped with cotton. All the above 5 glasses placed in hot-air closet where the temi)crature is kej)t by a self-regulating gas-jet at 33®-35® C. 8t/(, 10.30 A. M. I’l'inp. of closet 35.®1 C. The marked glass and the llask containing urine examined, eighteen hours after beginning ot expenment. Urine Tn marked glass.—Turbid. A white precipitate in apex of wineglass.](https://iiif.wellcomecollection.org/image/b22332510_0008.jp2/full/800%2C/0/default.jpg)
