Abel's laboratory handbook of bacteriology / [Rudolf Abel].
- Abel, Rudolf Velentine Ludwig, 1868-
- Date:
- [1912?]
Licence: In copyright
Credit: Abel's laboratory handbook of bacteriology / [Rudolf Abel]. Source: Wellcome Collection.
Provider: This material has been provided by The University of Leeds Library. The original may be consulted at The University of Leeds Library.
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![with four lots of water, draining carefully between each, and after the last washing allow the flask to stand inverted to drain for 10 minutes. Now add 500 c.c. of the meat extract, peptone, and salt, to the washed agar in the flask ; and place in the autoclave at 115° C. for 30 minutes, or in the steamer for 90 minutes; then add remainder of the meat extract, &c. Make alkaline with Liq. potassi (till blue litmus- paper is rendered slightly more blue), cool to 60° C, add the beaten white of an egg, heat in the autoclave for 45 minutes, or in the steamer for 90 minutes. Filter through a moistened Chardin's filter-paper (32 cm.) placing the funnel in a warm Avater jacket, put in tubes and steam the latter for 30 minutes on two successive days.] Modifications in the preparation of Broth, Gelatine, and Agar. 1. The meat extract may be diluted up to about four times its bulk without interfering with its nutritive capacity. Meat extract may also be made from flesh of animals other than the ox or horse, and may be pre- pared from i^lacenta, testicles of bulls (very cheap), &c. 2. Instead of meat extract prepared in the manner described above, Liebig's extract of meat (Lemco) may be employed in 1-2 % solution, in which case the addition of salt is unnecessary. The medium thus made from Lemco, however, is not so valuable as that made from meat in the manner described. For icater examinations the following gelatine preparation has been officially recommended :-Dissolve Lemco 2, 0 2](https://iiif.wellcomecollection.org/image/b21509645_0033.jp2/full/800%2C/0/default.jpg)
