Clinical diagnosis : a practical handbook of chemical and microscopical methods / [W.G. Aitchison Robertson].

  • William George Aitchison Robertson
Date:
1896
Catalogue details

Licence: Public Domain Mark

Credit: Clinical diagnosis : a practical handbook of chemical and microscopical methods / [W.G. Aitchison Robertson]. Source: Wellcome Collection.

    61/380 (page 53)
    Fallacies—Alcoho], ^ ^^^^^^ production of ^^^B'^^'' I yellow colour. Phosphates - If so, extract free lactic acid by shaking up with ether ; decant off ethereal solu- tion. Evaporate off ether. Redissolve residue in water, and apply test to this. 3. Uffelmann's Reaction. Reagent—20 c.c. water. 10 c.c. solution of carbolic acid (4 per cent.). Add few drops perchloride of iron solution = amethyst-blue colour. 'Add gastric fluid = yellow colour if lactic acid present. Fallacies (as under No. 2). Quantitative Estimation. Proceed as in the Mintz-Boas' Method (p. 49). Titrate the ethereal extract of lactic acid against the decinormal alkaline solution. Calculation—1 c.c. decinormal soda solution = 0*009 grammes lactic acid. Therefore multiply the number of cubic centi- metres of standard soda solution used by 0*009 = amount of lactic acid in 10 c.c. gastric fluid. Multiply this by 10 = percentage. Lactic Acid. Present—(1) Carcinoma of stomach—alivaijs. (2) Various fermentations in the stomach.