Experimental manipulation of gene expression / edited by Masayori Inouye.

Date:
1983
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Experimental manipulation of gene expression / edited by Masayori Inouye. Source: Wellcome Collection.

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    Contents vii III. Construction of a Prototype Retrovirus Vector 159 IV. Rescue of Recombinant Genomes as Infectious Virus 162 V. Characteristics of Retrovirus-Mediated Transformation 164 VI. Useful Derivative Vectors 167 VII. Conclusions and Prospects 170 References 172 9. Use of Retrovirus-Derived Vectors to Introduce and Express Genes in Mammalian Cells Eli Gilboa I. Introduction 175 II. Organization of the M-MuLV Genome 176 III. Use of Retrovirus Vectors to Study the Mechanism of Gene Expression of the M-MuLV Genome 178 IV. A General Transduction System Derived from the M-MuLV Genome 181 V. Summary and Prospects 187 References 188 10. Production of Posttranslationally Modified Proteins in the SV40-Monkey Cell System Dean H. Hamer I. Introduction 191 II. SV40 Late-Replacement Vectors 192 III. Human Growth Hormone 193 IV. Hepatitis В Surface Antigen 200 V. Conclusions and Prospects 207 References 209 11. Adenovirus Type 5 Region-El A Transcriptional Control Sequences Patrick Hearing and Thomas Shenk I. Introduction 211 II. Deletion Mutations in the 5'-Flanking Sequences of Ad5 Region EIA 213 III. Analysis of Mutagenized Templates in Cell-Free Transcription Extracts 214 IV. Analysis of Cytoplasmic EIA mRNAs Found in Vivo after Infection with Deletion Mutants 216 V. 5'-End Analyses of EIA mRNAs Synthesized in Vivo after Infection with Deletion Mutants 218 VI. EIA Transcriptional Control Region and Comparison to Other Eukaryotic Control Regions 219 References 222