Experimental manipulation of gene expression / edited by Masayori Inouye.

Date:
1983
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Experimental manipulation of gene expression / edited by Masayori Inouye. Source: Wellcome Collection.

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    Contents Contributors xi Preface xiü 1. Use of Phage X Regulatory Signals to Obtain Efficient Expression of Genes in Escherichia coli Allan Shatzman, Yen-Sen Ho, and Martin Rosenberg I. Introduction 2 II. Expression of Prokaryotic Gene Products 2 III. Expression of Eukaryotic Genes 8 References 14 2. Мик1риф08е Expression Cloning Vehicles in Escherichia coli Yoshihiro Masui, Jack Coleman, and Masayori Inouye I. Introduction 15 II. pIN-I Vectors 17 III. pIN-II Vectors 21 IV. pIN-III Vectors 23 V. pIM Vectors; High-Copy-Number Vectors 24 VI. pIC Vectors; Hybrid Expression Vectors 26 VII. Promoter-Proving Vectors 28 VIII. General Cloning Strategy 30 IX. Summary 31 References 31 3. Molecular Cloning in Bacillus subtilis David Dubnau I. Introduction 33 II. Plasmid Transformation 34 III. Plasmid Vectors 36 IV. Cloning Stratagems 45 V