Outlines of medical diagnosis : prepared for the use of students at the Harvard Medical School.
- Stone, Arthur K. (Arthur Kingsbury), 1861-
- Date:
- 1911
Licence: Public Domain Mark
Credit: Outlines of medical diagnosis : prepared for the use of students at the Harvard Medical School. Source: Wellcome Collection.
Provider: This material has been provided by the Francis A. Countway Library of Medicine, through the Medical Heritage Library. The original may be consulted at the Francis A. Countway Library of Medicine, Harvard Medical School.
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![BLOOD. Examination of Fresh Blood. Size, shape and color of red blood corpuscles (erythrocytes). Leucocytes, Fibrin, Parasites (Malaria Filariasis, etc.). Haemoglobin. Use Tallqvist's scale. This determines at once the presence or absence of anaemia. For more accurate estimations use a Fleischl-Miescher or Sahli apparatus. ^ , . . Per cent Haemoglobin ion Color mdex = —— _ , ^^^]^^^— ioo% Per cent Red Corpuscles — S'OOO'OOO Examination of Stained Specimen. Preparation of specimen, i. Cleanse the cover glass thoroughly v/ith soap and water. Wipe dry. 2. Wash the edge of the ear with water, and thoroughly dry. 3. Quickly pierce the lower edge of the lobe of the ear with a clean surgical needle. 4. Wipe away the first few drops of blood. 5. Touch the centre of the cover glass, held by its edges, against the top of the drop of blood, avoiding the skin. i 6. Drop this cover glass upon another clean cover glass. The blood should spread evenly between the two without any pressure. 7. Draw the cover slips apart, keeping their faces parallel. The blood films thus should dry almost imme- diately, and are then ready to be stained. Method of Staining, i. Cover the specimen with five or six drops of Wright's modification of Leishman's stain for one minute. 2. Add two or three times the quantity of water, and let this diluted stain remain for two minutes, for malarial organisms, four minutes. 3. Wash in water until the preparation has a pinkish color. Extra washing with water tends to decolorize the basic staining elements and to bring out the red acid stain. It is useful in bringing out the red granules in malarial organisms. 19](https://iiif.wellcomecollection.org/image/b21167539_0037.jp2/full/800%2C/0/default.jpg)
