On the modifications of the metabolism produced by the administration of diphtheria toxine / by D. Noel Paton ... [and others].

Date:
[1899]
Catalogue details

Licence: Public Domain Mark

Credit: On the modifications of the metabolism produced by the administration of diphtheria toxine / by D. Noel Paton ... [and others]. Source: Wellcome Collection.

Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.

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    Sulphuric acid was estimated by heating 50 c.c. of the urine with hydrochloric acid and adding barium chloride. The precipitate was washed and weighed. The total sulphur was estimated as sulphuric acid by igniting with caustic potash and nitre, dissolving the ash with hydrochloric acid and precipitating with barium chloride. The sodium and potassium were determined as follows:— 100 c.c. of the urine were acidified with hydrochloric acid and 2grms. of carbonate of calcium were added. Ammonia was next added and the mixture heated and filtered. Oxalate of ammonia and ammonia were added to the filtrate and it was heated and filtered. The filtrate was evaporated to dryness in a platinum basin and ignited. The ash was dissolved in water and filtered. To the filtrate a solution of strontium chloride in alcohol was added, and it was heated and filtered. The filtrate was then heated to remove the alcohol, and an excess of carbonate of ammonia was added, and it was heated and filtered. The filtrate was dried in a platinum capsule ignited, dissolved in water and the treatment with carbonate of ammonia repeated, and after re- evaporation the residue was dissolved in water, a few drops of hydro- chloric acid added, and the fluid was evaporated to dryness in a platinum capsule and weighed. This gives MgCl2 + KC1 + NaCl. The residue was then dissolved in water with a little hydrochloric acid, made up to 100 c.c. and divided into two equal portions A and B. In A the magnesium was estimated by the phosphate of soda method. From the Mg2P207 the MgCl2 was calculated and, deducted from the half of the first weighing, gave the KC1 + NaCl. To portion B, platinic chloride in excess was added—the solu- tion being highly-coloured, by excess of platinic chloride. It was evaporated nearly to dryness in a water-bath and 80°/0 alcohol was added. After standing for three hours a crystalline precipitate formed. This was washed with a mixture of alcohol and ether. The precipitate was dissolved in hot water, and the process repeated. The precipitate was collected on a dried weighed filter-paper, washed with alcohol and ether, dried and weighed. This gave 2KC1 + PtCl4. From this KC1 was calculated and by subtraction gave the NaCl. B. Fceces. The faeces were weighed, treated with 10 per cent, sulphuric acid and dried. The nitrogen was determined by Argutinsky’s modification of KjeldahPs method.
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