"Supercoiled DNA"

Date:
1978-1979
Reference:
PP/CRI/H/6/12/3
Catalogue details

Licence: In copyright

Credit: "Supercoiled DNA". Source: Wellcome Collection.

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    VI. Application (relationship) to DNA. A. For mechanical properties must consider various other properties such as Shear, etc. B. Here consider only Lk. Exact definition of ribbon not necessary. Consider various approximate definitions. i) Ribbon curved for DNA. Reject as inconvenient, ii) Ribbon whose axis coincides with DNA axis. C. Edges of ribbon: lie at some ^Infinitesimal) points perpendicular to (pseudo) dyad axis. N.b. cross sectional diagram. VII. Relationship to experiment for supercoiling, etc. A. Results of electron microscopy. B. Buoyant separation in dyes. C. Gels i) Explanation in terms of ALk. ii) N/C enzyme and how prepared, iii) Thermal envelope. Gaussian. C. Bands melted and rerun show identical pattern. Excise 1 band and treat with N/C enzyme: regenerate pattern. VIII. Native viral supercoiled DNA and nucleosomes. A. Coiled on cylinder. B. Handedness. C. Cite histones and SV40 I + N/C*I. o D. Consider self-linking analysis in regard to nucleosomes. Present as another way to view the matter.
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