Report of the Departmental Committee apointed to inquire as to precautions for preventing danger of infection from anthrax in the manipulation of wool, goat hair, and camel hair.
- Great Britain. Committee on Anthrax.
- Date:
- 1918
Licence: Public Domain Mark
Credit: Report of the Departmental Committee apointed to inquire as to precautions for preventing danger of infection from anthrax in the manipulation of wool, goat hair, and camel hair. Source: Wellcome Collection.
46/356 page 38
![Treatment of Test Material.—In eight of the earlier experiments the natural clots were placed among wool in nets, which were put among loose wool in the baths. The preliminary treatment was divided into two periods of 15 minutes, during which the test material was immersed in water at a temperature of 102—104° F. and squeezed through rollers at the end of each period. The disinfection treatment consisted of immersion in a 23 per cent. solution of formaldehyde at temperatures ranging from that of air (in two experiments) to 101° F. (in six experiments). The duration of this stage was 15 minutes in four, 30 minutes in two, and 60 minutes also in two experiments. ‘The test material was squeezed through rollers at the end of these periods, and then dried in a current of air at temperatures of 165-212° F. In the remaining two experiments of Group 12 the clots were placed loose among wool in the baths and the periods of the preliminary treatment in water were reduced to 10 minutes each. The disinfection part of the process consisted of immersion for two periods of 10 minutes each in a solution of formaldehyde of 2% per cent. strength at a temperature of 102° F. In both stages the material was squeezed through rollers at the end of each period. In one experiment the test material was dried at 157° F., and in the other it was not dried. Results ——The results of these experiments can only be judged by the condition of the clots after treatment and by the number of organisms other than anthrax it was possible to cultivate from them. The condition of those clots placed among wool in nets for treatment was very unsatisfactory, as there were often masses of other substances as well as of blood only partially disintegrated. The number of organisms surviving the treatment was large in five out of the eight experiments in which this course was followed. Excluding these, however, and considering only the two experiments in which the clots were placed loose in the baths, the number of organisms which survived the treatment was as great as in the experiments of Group 8, in which artificial clots were used. The experiments of Group 12 showed, in fact, exactly the same defect in the preliminary treatment as was indicated in the first eight groups. EXPERIMENTS OF Group 13. Table 13.—Experiments with Natural Bloodclots. Process :—(1) Treatment in warm soap solution. (2) Squeezing through rollers. (3) Treatment in warm formaldehyde solution. (4) Squeezing through rollers. (5) Drying in a current of hot air. (6) Disinfection allowed to continue (t.e., ammonia solution not added to test material to destroy formaldehyde). } ¢ | x Ha} Treatment in Soap Treatment in Drying in Current) 2 3 Number of | $ poe Sots use Solution. Formaldehyde Solution. of Hot Air. 5 = Colonies ont < | o£ | growingon | 5 @ 5 | l uf l an | oy |Agarat37°C.) © & a &0 ohaiee il =I ae Fe ] ae = | aie Pike teeny | agree = 2 | from oof | 28 S| ty aa See Rae {meet es en eos & |Materialfrom; 5 ° a, 2 3 a = 3 no} | g 5 aa 4 i 8 ar oes ae a 8 R= | 4 je = 8 | treated Clots. ap : : e° | 2 | 2 gees 2 | sieeice) S| es ae ce S f Sp See eee) Ot ee heey aa) oe .| Se ; 3 : 2 | |slado, @ |e |g@/ e912) | oe] w lee] ss i Zz, S g om [Paul g Os Re hg O° a os On A q o R eS Oo} o H° eS © Bx x a2 oo < H RQ © = Wreaere y[s) oH 3 s ogig Bil ie tba eae ee ET oe et Ba Nes 2 ma | oe a Paes ie <q =a °F, [Mins.| & ~ | °F. |Mins.| |< | °F, |Mins.| Hours. © |Hours. (1) | @) | @) | ©] G/| OO] @M | & | (G9] aD | G2)! d3){ a4) |] a5) | (6) 7 a7) Half of a |) a] | natural | ; very heavily! | s . 135 |< bloodstained| Loosein |!110 | 40} 3 | 2:51 |. 102.) 20422) | Loose! 1561.45 142 |; — Qs. em | Bagdad skin baths | | on. fleece ; not) \ together \ | grids. | re | infected. | f with other) ‘ | / | ) ( Large, thick! clots | f | | | mohair clot! | (natural). | | | | | 136 |{ picked out) | . 5 | | 45 5 ‘ ||. by sorters =| 110 | 40) 43 2251 es 20 | 2 nO 156 | 45 | 142 = 0 pa | | not infected. J | | { Large } ) | natural | | infected / ~ |} bloodelot So ery Gs ae 137 is picked out | | LLD S40 ee Sate 2e2O Re LOS n a2 O mms, weuie LODE oes 69 ; 0 Bie | of Bagdad | | | | | {skin wool, | | | | ben id | 138 ms | | Together || 115 | 40) 3 | 2°29) 98| 20] 2 » | 166] 23 69 | 0 1; — Be in baths | ‘ | . ) | ( Large | f loose with} / natural other clots // | bloodclot (natural). | |} picked out | lenis vneiee 139 4 of Bagdad | 116 1540) 3. 52°29) 98) 20 sero » | 166} 23) 69 | — ib | skin woo! ; ' | not : | | | | { infected:, «| ' oe, ed a '](https://iiif.wellcomecollection.org/image/b32178049_0046.jp2/full/800%2C/0/default.jpg)
