Volume 1
A textbook of pathology : systematic & practical.
- Hamilton, David James.
- Date:
- 1889-94
Licence: Public Domain Mark
Credit: A textbook of pathology : systematic & practical. Source: Wellcome Collection.
Provider: This material has been provided by Royal College of Physicians, London. The original may be consulted at Royal College of Physicians, London.
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![cation d. Anilin-farbstoffe, Wien, 1883. Ehrlich: Arch. f. mik. Anat., xiii. p. 263 ; Charite-Annalen, 1886 ; Zeitschr. f. 'vvissenscli. Mikroskoj^ie, u. f. inik. Technik, iii. 1886, p. 525. Gierke : Zeitschr. f. wissensch. Mikroskopie u. f. mik. Technik, ii. 1885, p. 13. Griesbach (Azofarbstoffe) : Arch. f. mik. Anat., xxii. 1887, p. 139. Hankin (Aniline Dyes); Quart. J. Mic. Sc., xxvii. 1886, p. 401. Hummel: The dyeing of Textile Fabrics (Manuals of Technology, ed. by Ayrton and Wormell), London, 1885. Jaquemin: Comptes rend., Ixxviii. 1874, p. 1306. Schultz: Chemie d. Steinkohlentheers, etc. 1882. Reeves (Howto Fix Aniline Dyes): Brit. Med. J., 1883, i. p. 450. VI. Nitrate of Silver.—In staining endothelia of serous membranes, of blood-vessels, or of lymphatics, or in elucidating the structure of the cornea, there is no reagent to be compared to silver. It jDossesses this disadvantage, however, that it will stain the tissue only when it is living or immediately after the animal has been killed. It is conse- quently only in experimental pathology that it is of any use, or in the staining of recently excised parts such as tumours, etc. For the experimental study of inflammation it is quite indispensable. It has the property of staining the cement substance between endothelial cells, and hence blackens their outlines. In artificial keratitis it demarcates the inflamed from the sound parts very graphically, by showing the broken up endothelium of the plasma canals. In peri- tonitis it shows the germinating cells derived from the endothelium and the leucocytes exuding from the blood-vessels. The best strength is from ^ to p>er cent solution in distilled water. This is to be dropped on to the part until it becomes milky, the excess washed otf with care in distilled water, and the preparation, immersed in a mixture of equal parts glycerine and distilled water, allowed to become brown in the light but not in the direct rays of the sun. Care should be taken not to overstain the tissue, as all silver prepara- tions tend to become too black by keeping. The cornea should not be placed in glycerine and water but in dilute acetic acid. After twenty-four hours, it will be found to have swollen to twice or three times its natural size, and can then be easily split into lamellae. The endothelium of lymphatic vessels in a transparent tissue, such as the central tendon of the diaphragm in the rabbit and guinea-pig, can be readily stained by dro])ping on a | to ^ per cent solution, and subse- quently washing in distilled water. VII. Terchloride of Gold.—This is open to the same objection as silver, namely, that in order to be satisfactory the staining must be done in a perfectly fresh tissue. A great deal of uncertainty is at- tached to this method of staining, chiefly due to the different qualities of gold chloride which are to bo purchased. There seems to be an impurity in some of them which invalidates their power of staining. The pathological tissues for which it should be employed are the artificially-inflamed cornea, tendon, or cartilage, or, in fact, any inflamed connective tissues. It is not to be recommended for the central nervous system as its action is so uncertain, and as all its best effects can be brought out by Weigert’s hsematoxylene stain (Sect. 43).](https://iiif.wellcomecollection.org/image/b24990607_0001_0116.jp2/full/800%2C/0/default.jpg)
