Volume 1
A textbook of pathology : systematic & practical.
- Hamilton, David James.
- Date:
- 1889-94
Licence: Public Domain Mark
Credit: A textbook of pathology : systematic & practical. Source: Wellcome Collection.
Provider: This material has been provided by Royal College of Physicians, London. The original may be consulted at Royal College of Physicians, London.
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![The section to be mounted in it should be taken out of water in the same manner as that formerly recommended. All the water should be removed from the slide close up to the preparation, and as much as possible from the preparation itself without drying it. A drop of the medium is now allowed to fall upon it, care being taken not to churn up air-bells in the bottle by drawing the glass rod out too quickly. It is next covered with the cover slip, pressed gently down, the superfluous medium removed with a soft rag, and left in a dry place for ten days to a fortnight before being cemented. (2) Cam])lior Mounting Fluid (Hamilton).—In order to avoid the de- composing action of the arsenious acid upon various metallic stains a mixture made virtually on the same principle as Farrants’ solution, but containing camphor instead of arsenic, may be substituted. The receipt is the following :— Camphor water ...... 2 parts. Glycerine. . . . . . .1 part. Saturate with picked gum acacia as in making Farrants’ solution, and filter through paper. Keep in a stopper bottle with several small pieces of camphor floating in it. It is chiefly useful for Prussian blue injections. (3) Iodine Mounting Fluid (Hamilton).—This is employed for mount- ing sections of waxy organs stained in iodine. The section is stained in the manner described under staining fluids, and a large drop of the fluid is placed over it. A cover-glass is adjusted, but should not be pressed down. It, like the camphor medium, is made essentially in the same way as Farrants’ solution, but iodine is added instead of arsenic or camphor. Take of Glycerine . . . • . 1 oz. „ Water . . . . . . 2 „ „ Liquor iodi (Phar. Brit.) . . . 4 „ Saturate with picked gum acacia as before and filter through paper. Keep in a stopper bottle. (4) Glycerine Jelly (Hamilton).—To make a good glycerine jelly for microscopic purposes, there should be as little water in its composition as is consistent with the melting and subsequent consolidation of the o-elatine. What one desires in this medium is as much glycerine as possible with just sufficient gelatine to cause it to gelatinise. A quantity of French gelatine is soaked in water until it becomes pliable (about ten minutes). The water is roughly run off from it, or it may be })artially dried in a towel. It is next melted, and 15 ounces of this and 30 ounces of glycerine arc measured out. The white of an egg is beat up with half of the glycerine, while 1 ounce of liquid carbolic acid is added to the other half. The gelatine is allowed to cool as much as possible, without actually causing gelatini- sation. The glycerine containing the white of egg is then added.](https://iiif.wellcomecollection.org/image/b24990607_0001_0122.jp2/full/800%2C/0/default.jpg)
