Technique of tissue culture "in vitro" / by T.S.P. Strangeways.

  • Thomas Strangeways
Date:
1924
Catalogue details

Licence: In copyright

Credit: Technique of tissue culture "in vitro" / by T.S.P. Strangeways. Source: Wellcome Collection.

    96/104 (page 78)
    placing it, culture-side upwards, on the glass slide. Differen- tiation must be continued until the cells are clear and the nucleoli or the chromosomes of the cells in mitosis if present, stand out clearly. (12) When the culture is sufficiently differentiated wash in running distilled water for 5 minutes. (13) Mount directly into Farrant’s mounting medium and label. If preferred, the specimen may be passed through the alcohol and xylol and mounted in Canada balsam. SECTION XLV To Stain Cultures for Fat (1) Proceed as described in Section XLV., but after differentiation and washing in distilled water place the specimen in a solution of Scharlack R (Section XLIII.) for 5 minutes, or until examination under the microscope shows the fat is well stained. (2) Wash in distilled water. (3) Mount in Farrant’s mounting medium. SECTION XLVI To Prepare Bouin’s Picro Formol Solution Picric acid saturated solution in distilled water 75 parts, formol 25 parts, glacial acetic acid 5 parts. SECTION XLVI I Fixation of Cultures by Bouin’s Solution and Staining with Iron Hematoxylin One per cent, solution of hsematoxylin in distilled water, solution of iron alum, Bouin’s picro-formol solution, absolute alcohol, xylol, Canada balsam, distilled water, saline solution, glass slides, fine forceps, watch glasses, glass capsules, Petri •dish, labels, small scalpel.