Collected papers on trypanosomiasis / by Sir David Bruce and others.

  • David Bruce
Date:
[1909-1911]
Catalogue details

Licence: Public Domain Mark

Credit: Collected papers on trypanosomiasis / by Sir David Bruce and others. Source: Wellcome Collection.

    24/268 (page 369)
    The method of carrying out these experiments was always the same. The tlies were fed for some days on a highly infected monkey, whose blood on microscopical examination was seen to contain numerous trypanosomes of Sleeping Sickness, and afterwards on a series of healthy monkeys. The following table gives the number of flies used in each experiment, the number of days they were fed on a monkey whose blood contained Trypano¬ soma gambiense, the number of days which elapsed before the flies became infective, and the number of days the flies remained infective. The minus signs signify that the flies failed to become infected, or at least failed to O O t/ f infect; or, in other words, that the experiment was negative. Table I.—Development of Trypanosoma gambiense in Lake-shore Glossina pcdpalis. 1 V Experiment. No. of flies. No. of days fed on. No. of days before flies became infective. No. of days flies remained infective. 624 60 3 656 280 3 — — 663 60 2 18 75 676 500 3 29 47 721 50 3 — — 9S0 350 3 — — 986 100 2 19 35 987 50 2 40 51 989 50 2 45 45 1020 100 3 — — 1023 50 3 37 51 1026 70 3 31 48 1198 20 40 — — 1372 100 3 — — Of these 14 experiments, seven are positive and seven negative. In the positive experiments 880 Hies were used, an average of 126; in the negative 960, an average of 137. The shortest time which elapsed before a fly became infective was 18 days, the longest 45 days, and the average 32 days. It may be well to give some of these experiments more in detail, in order to show the methods used, and draw attention to various interesting points. Experiment 624. To ascertain if development of Trypanosoma.\ gambiense takes place in the alimentary canal of Wild or Lake-shore Glossina palpalis. March 31, 1909.—Two batches of Glossina palpalis, caught on the Lake-shore, consisting of 30 Hies in each batch, were fed to-day on a monkey whose blood contained numbers of Trypanosoma gambiense.