The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1905
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.
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![751. Picronigrosin.^MARTiNOTTi {loc- cit., 1884, p. 478) stains for two oi' three hours or days in a saturated solution of nigrosin in saturated solution of picric acid in alcohol, and washes out in a mixture of 1 part of formic acid with 2 parts of alcohol until the grey matter appears clearly differentiated from the white to the naked eye, 752. Kaiser {Zeit. wiss. Mik., vi, 1889, p. 471) stains Bections of spinal cord for a few hours in a solution of 1 pai-t of naphthylamin brown, 200 of water, and 100 of tilcoliol, washes with alcohol, clears with origanum oil, and mounts. 753. Alizarin.—Scheottee [Neurol. Centralh., xxi, 1902, p. 338; Zeit. wiss. Mik., xix, 1903, p. 381) stains sections for twenty-four hours in a 1 to 2 per cent, solution of sul- phalizarinate of soda, differentiates for ^ to 1 minute in tap-water^ dehydrates^ and mounts. This is a general stain, but demonstrates Nissl bodies and other internal details. 754. Woltee's Chloride of Vanadium process {Zeit. wiss. Mik., vii, 1891, p. 471) : The material (either central or peripheral nervous tissue) is hardened in the hichrornate liquid of Kultschitzky, § 59, followed by alcohol, as there described. Sections are mor- danted for twenty-four hours in a mixtu.re of 2 parts of 10 per cent, solution of chloride of vanadium and 3 parts of 3 per cent, solution of acetate of aluminium, washed for ten minutes in water, and stained for twenty-four hours in a solution of 2 grammes of hsematoxylin (dissolved in a little alcohol) in 100 c.c. of 2 per cent, acetic acid.. They are washed out until they are of a light blue-red colour in 80 per cent, alcohol acidulated with 0-5 per cent, of hydrochloric ' acid. Remove the acid thoroughly by washing with pure alcohol, dehydrate, clear with origanum oil, and mount. Chiefly an axis-cylinder stain, myehn being coloured only if the differentiation in the acid alcohol is insufficient, but cells are also stained. 755. ScAEPATETTi (Neurol Centralh., xvi, 1897, p. 211; Zeit. toiss. Mi]c.,xiv, 1897,p.91) obtains an axis-cylinder and cell-stain as follows:— Sections of formol material are stained for fire minutes in 1 per cent, hematoxylin, treated for five minutes with concentrated solution of neutral acetate of copper, difeerentiated with Weigert's borax-fern-](https://iiif.wellcomecollection.org/image/b21462586_0420.jp2/full/800%2C/0/default.jpg)
