The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1905
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.
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![pheral nerves as well as to nerve-centres, and also to the study of lymphatic glands, skin (see Schieffeedeckee, Anat. Aiiz., ii, 1887, p. 680), bile-capillaries, and other objects. The process is applicable to tissues that have been hardened in alcohol or in any other way, provided that they be put into a solution of a chromic salt until they become brown, before mordanting them in the copper solution. It is not necessary that the mordanting be done in bulk. Max Flesch {Zeit. wiss. Mik., iii, 1886, p. 50) prefers (following Lichtheim) to make the sections first, and mordant them separately. Vassalb (quoted from Baton's Hist. Untersuchungsmeth. d. Nerven- sijstems, Wiirzbiu-g, 1905, p. 124) first stains the sections in 1 per cent, hsematoxylin, for tlu-ee to five minutes, puts for thi-ee to five minutes into saturated solution of acetate of copper, and differentiates. For a method for regenerating the staining solution after use, see Fanny Bbrlinekblau, Zeit. wiss. Mik., 1886, p. 60, or early editions. Paneth {ibid., 1887, p. 213) makes the stain with extract of logwood instead of pure hsematoxylin. Breglia {ibid., vii, 1890, p. 236) stains with liquid extract of logwood or Pemambuco wood. For both of these see early editions. Gekota (Intern. Monatsschr. Anat., siii, 1896, pp. 138, 139 ; Zeit. wiss- Mik., xiii, 1896, p. 315) states that the reaction can be obtained by using the copper after the stain, and that an alum-hsematoxylin may be used. 785. Weigeet's 1891 Method [Deutsche med. Wochenschr., 42, 1891, p. 1184; Zeit. wiss.'3Iik., viii, 1891, p. 392).—The material is to be hardened in bichromate and imbedded in celloidin (see last §). Then in the original form of this process Weigert proceeds as follows : The hardened blocks of celloidin are brought into a mixtui-e of equal parts of a cold sattu-ated solution of neutral acetate of copper and 10 per cent, aqueous solution of potassio-tai-trate of sodiiun (C4H406KN'a + 4H2O, salt of Seignette). They are left in the mixture for twenty-four hours in an incubator. (Large specimens [pons] will require forty-eight houi-s, the mixture being changed for fresh at the end of twenty-fom- hoiu-s.) They are then brought for twenty-four hours into aqueous solution of neutral acetate of copper, either saturated or diluted with 1 volume of water, being kept as before in the incubator. They are then rinsed with water and brought into 80 per cent, alcohol, in which they may either remain till wanted or be cut after half an hoiu-. The object of the salt of Seignette was to prevent the pure cupric acetate from forming precipitates on the tissues. Weigert now {EncycL mik. Technik., 1903, p. 942) has](https://iiif.wellcomecollection.org/image/b21462586_0436.jp2/full/800%2C/0/default.jpg)
