The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1905
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.
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![CHAPTER XXXIII. Modifications of Weigert's Method. 786. Pai/s Method, {Wein. med. Jahrb., 1886; Zeit. wiss. Mih., iv, 1887; p. 92; Med. Jahrb., 1887, p. 589; Zeit. wiss. Mile, 1888, p. 88).—This is a chrome-lake process. You proceed at first as in Weigert's process, but omitting the copper hath, and you stain as in Weigeet's process. After staining in the haematoxylin solution the sections are washed in water (if they are not stained of a deej) blue a trace of lithium carbonate must be added to the water). They are then brought for twenty to thirty seconds into 0*25 per cent, solution of permanganate of potash, rinsed in water, and brought into a decolouring solution composed of— Acid. Oxalic, pur. . . . . 1*0 Potassium Sulphite (Kalium Sulfuro- sum [SO3K2]) .... 1-0 Aq. Dest. 200-0 In a few seconds the grey substance of the sections is decolourised, the white matter remaining blue. The sections should now be well washed out, and may be double-stained with Magdala red or eosin, or (better) with picro-carmine or acetic-acid-carmine. For further details see the papers quoted, or Behrens, KossEL, and Schiepferdecker's Das Mih-oskop, i, p. 199. Pal's process gives brilliant results, the ground of the preparations being totally colourless. But it has the defect that the differentiation is more rapid than is desirable. The whole process of differentiation only lasts some seconds, so that an error of judgment of only a few seconds may entirely vitiate the result. Weigert {Ergehnisse, vi, p. 21) considers that for very thick sections the process is superior to his own. But it is not so safe for very fine fibres, and is not applicable to his collodion series method ; each section must be treated sepa- rately. Marcus stains by the Pal metliod sections of material hardened in formalin, as described § 745. Gtjdden {Neurol. Centralb., xvi, 1897, p. 24) makes celloidin sections of material hardened in 5—10 per cent, formol followed by alcohol, * Not sulphide, as erroneously given in Mercier's Les Coupes du Systeme Nerveux Central, p. 190.](https://iiif.wellcomecollection.org/image/b21462586_0438.jp2/full/800%2C/0/default.jpg)
