The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1905
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.
444/572 page 430
![wiss. Mih., XX, 190-i, j). 458) descrilses a method of treating Marchi material that seems to me to be of inadmissible complexity. 801. Iron.—Allerhand {Neurol. Centralh., xvi, 1897, p. 727; Encycl. mih. Technih., p. 944) puts sections of Miiller material for fifteen minutes into warm 50 per cent, solution of Liquor ferri sesquichlorati, then for an hour or two into 20 per cent, tannin solution (old and brown). They are then differentiated by the method of Pal, taking, however, the liquids twice as strong. An iron-alum process is described by Strong in Journ. camp. Neurol., xiii, 1903, p. 291. 802. Silver Nitrate.—Yastarini-Cresi {Ait. Accad. Med.-Chir. Napoli, 1,1896) hardens in formol, cuts thick sections, washes them with 40 per cent, alcohol, puts them in the dark into 1 per cent, solution of nitrate of silver in alcohol of 40 per cent, to 70 per cent., then washes thoroughly. Similarly, MossE {Arch. mih. Anai., lix, 1901, p. 401) imirregnating bichromic material with 1 per cent, solution of argentamin, and reduc- ing in 10 per cent. pyrogaUic acid, and differentiating by the method of Pal. 803. Gold Chloride for Peripheral ITerves (Frey, Arch. Anat. Phys. Anat. Ahth. Supp., 1897, p. 108. See Grundziige, Lee & Mayer, p. 421). 804. Polarisation.—Myelin can sometimes be detected in fresh material by the polariscope (see Ambronn & Held, Ber. Math. Phys. Ges. Wiss. Leipzig., 1895, p. 37). They examined teased preparations of peripheral nerves fresh in normal salt solution, or thick sections of nei-ve- centres cut with the freezing microtome. See also Gad & Heymans, Arch., Anat. Phys., Phys. Ahth., 1890. p. 531. Myelin-and-axis-cylinder Stains. 805. Palladium (Paladino, Rendic R. Accad. Scienze, Napoli, iv, 1890, p. 14, and 1891 [1892], p. 227; Zeit. wiss. Mih., vii, 1890, p. 237, andix, 1892, p. 238) .—Pieces of material hardened in bichromate, chromic acid, or corrosive sublimate, and not more than 5 to 8 mm. in thickness, are put for two days into a large quantity (at least 150 to 200 c.c. for each piece) of 01 per cent, solution of chloride of jDalladium (see § 82). They are next put for twenty-four hours into a solution of iodide of potassium of 4 : 100 strength, of Avhich](https://iiif.wellcomecollection.org/image/b21462586_0444.jp2/full/800%2C/0/default.jpg)
