The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1905
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.
454/572 page 440
![the processes necessary for imbedding, will not always resist those necessary for merely mounting in balsam, and even then may easily go bad after tliey have been mounted for a short time. A critical review of the Golgi metliod by Weigert may he found in Ergebnisse der Anatomic, v, 1895 (1896), p. 7. He thinks the precii^itate certainly consists of a silver chromate, but that we cannot say which. The method has also been critically studied by Hill {Brain, pai-t 73, 1896, p. 1). He thinks the stain depends on the formation of a reduced salt (subsalt) of silver, not of a silver chromate, and that the reduction takes place, not in the nervous fibrils, but in the liquid or semi-liquid neuroplasm with which they are bathed. He finds the impregnated material will stand imbedding in celloidin for many days. AzouLAY {Comptes Bend. Soc. Biol. [10], i, 1894, p. 839) has followed the process tmder the microscope, and holds that it is due to a ciystal- lisation of chromate of silver in the tissues. Kallitjs {Encycl. oniJc. Technih., p. 466) thinks that an albumino- chromate of silver is formed. Modifications concerning the Impregnation of the Tissues. 816. Eamon Y Oajal {^eit. wiss. Mile., vii, 1890, p. 332) gives 3 per cent, as the strength of the bichromate in the mixture for the rapid process, and in numerous other places has given it as 3-5 per cent. This latter strength has been adopted by most of the workers who use the rapid process, and the mixture containing this proportion of bichromate is generally known as Ramon y Cajal^s mixture. 816a. Ramon y Cajal's Douhle-Impregnation Process {Trah. Lab. Hist. Med. Barcelona, 1891; Zeit. iciss. Mik., ix, 1892, p. 239).—After hardening for three days (embryos of fowl) in the osmium-bichromate mixture the preparations are put for thirty-six hours into nitrate of silver solution (0'5 to O'To per cent.). They are then brought back for a day or two into the same osmium-bichromate mixture, or into a weaker one containing only two parts of osmic acid solution to 20 of the bichromate. After treatment with this they are washed quickly with distilled water, and put for a second time into the silver solution for thirty-six to forty-eight hours. It is important to hit off the proper duration of the first impregna- tion in the bichromate. If it has been too long (four days) or too short (one day), the second impregnation will not succeed. In this case a third impregnation must be resorted](https://iiif.wellcomecollection.org/image/b21462586_0454.jp2/full/800%2C/0/default.jpg)
