The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1905
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.
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![Odiee [La Rachicocainisation, Geneve^ 1903, p. 27) take!? two parts of undiluted formalin, instead of the 10 per cent. The formalin and bichromate mixtures should be kept in the dark. It is well only to make them up at the instant of using them. Odier finds these mixtures afford a more abundant impregnation, with fewer precipitates. KopscH {Anat. Anz., xi, 1896, p. 727) takes 4 parts of 3-5 per cent, bichromate solution, and ] of commercial formalde- hyde solution, and after twenty-four hours transfers to pure 3*5 per cent, bichromate. He finds that by this means precipitates are almost entirely avoided. Gerota {Intern. Monatsschr. Anat., xiii, 1896, p. 108) first hardens (brain) for a week or two in 5 to 10 per cent, formol solution, then puts small pieces for three to five days into 4 per cent, bichromate, then into the silver. Similarly Bolton {Lancet, 1898, p. 218 ; Journ. Roy. Mic. Soc, 1898, p. 244). ScHEEiBEE {Anat. Anz., xiv, 1898, p. 275) obtained good results (on appendages of Crustacea which were impervious to the osmic mixture) with mixtures of five parts 2-5 per cent, bichromate to one of 4 per cent, formaldehyde, or one part 2-5 per cent, bichromate to two of 5 per cent, formalde- hyde, the specimens remaining for one day in the first, for two days in the second. Similarly Duboscq {Arch. z. Exper., 1899, p. 483), warm- ing the mixture to 40° C. VAN Gehdchten {in litt.), and other observers, have not ohtained good results with formaldehyde. 820. Acetic Aldehyde.—Yassale and Donaggio {Monitore Zool., Ital., vi, 1895, p. 82) harden pieces of at most 1 cm. in tliickness for fifteen to twenty days in a mixtm-e of five parts of aldeliyde witli 100 of 3 to 4 per cent, bichromate, changing tlie fluid after a few days, as soon as it has become dark. The rest as Golgi. 821. Veeatti's Liquid.—For the study of Golgi's endo- cellular network Golgi {Verh. Anat. Ges., xiv, 1900, p. 174) uses a mixture due to Yeeatti, consisting of : 5 per cent, bichromate . . .30 parts. 0-1 per cent, platinum chloride . . 30 „ 1 per cent, osmic acid . . 15 to 30 „ and after hardening therein puts (for a time varying from a](https://iiif.wellcomecollection.org/image/b21462586_0456.jp2/full/800%2C/0/default.jpg)
