The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1905
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.
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![an equal volume of 10 per cent, formol; pieces of brain or spinal cord are put into the mixture for five days, and are then brought direct into a mixture of equal parts of 10 per cent, formol and hydric sulphide solution. After five days therein they are ]3assed through successive alcohols, im- bedded in celloidin, cut, and the sections mounted in xylol- balsam under a cover. They seem to be quite permanent. Nerve-cells as well as nerve-fibres are impregnated. The impregnation is a very comj)lete one. OoENiNG {Anat. Anz., xvii, 1900, p. 108) hardens the tissues with 10 per cent, formol before bringing them into the formol-formate mixture, and so obtains better results. He obtains his formate of lead direct from Merck {PLumhum formicicum). He thinks the celloidin imbedding injurious, and prefers to cut without imbedding. He prefers to clear sections with clove oil. The method appears to him par- ticularly useful for the medulla oblongata, with which the Golgi method does not succeed. Other details loc. cit. 840. Methylen Blue for Central Ifervous System (Semi Meyer, Arch. mih. Anat, xlvi, 1895, p. 282, and xlvii, 1896, p. 734).—Meyer has obtained good results (for the central nervous system, not for tlie peri- pheral) by means of suicutaneous injection. Large quantities of solution must be injected. A young rat wiU require at least 5 c.e. of 1 per cent, solution; a rabbit of a fewweeks abotit 40 c.c. But it is laetter to employ stronger solutions, 5 to 6 per cent. The total dose should be given in several portions, at intervals of one to several houi'S. It is not necessaiy to wait till death by intoxication has taken place, and after a suitable interval the stibject may be killed. It is not necessary to expose the organs to the air for the sake of oxydising the stain. They should be thi-own direct into the bath of Bethe, § 352. The liquid ought to be well cooled before use. The preparations should remain in it till the next day. Ramon y Oajal (Bev. trim. Micr., Madrid, i, 1896, p. 123; Zeit. iviss. ilKfc., xiv, 1897, p. 92) stains by propagation or difEusion. The brain is exposed (rabbit) and the cortex is divided into slices of a couple of millimetres thickness by means of a razor. The slices are then covered on both sides either with finely powdered methylen blue, orwith a saturated solution of the same, the slices are rej)iaced in their natiu-al positions, the brain-case is replaced for half an hour, after which the slices are removed and fixed for a couple of hoiu-s with Betlie's ammo- nium molybdate, washed, hardened for three or foiu- hom*s in a mixtm'e of 5 parts 1 per cent, platinum chloride, 40 parts formol, and 60 parts water, fm-ther treated for a few minutes with platinum chloride in alco- hol (1 in 300), and if small enough imbedded in parafl&n. The sections](https://iiif.wellcomecollection.org/image/b21462586_0464.jp2/full/800%2C/0/default.jpg)
