The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1905
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.
466/572 page 452
![NEUROGLIA, AND NERVE-END ORGANS. Neuroglia. 843. Introduction.—Neuroglia cells may be isolated by teasing, and may be stained in many ways (see Ranviee, Traits, p. 1063), by osmic acid, lisematoxylin, carmine, orcein. But by far the best method for the study of the forms and relations both of ependyma cells and astrocytes is the Bichromate-and-silver Impregnation of GtOlgi ; the best material being that which has been for not more than two or three days in the osmio-bichromic mixture. This method, however, does not tinctorially differentiate between neuroglia-cells and nerve-cells, and is of no use for the purpose of mapping out tracts of neuroglia as a whole. The following methods serve for this. They are such as either stain neuroglia quite specifically, leaving all other tissues unstained (Weigeet) or such as stain it in a different tone to other tissues. Wbigeet's process stains the processes of the cells (his fibres ) intensely, whilst leaving the cell- body unstained, and in consequence, if exclusively followed, may lead to erroneous conclusions. 844. Weigeet's Specific Neuroglia Stain (Weigeet's Beitr. zur Kenntniss der normalen menschlichen Neuroglia, Frank- furt-a-M., 1895 ; and his art. Neurogliafdrhung in Encycl. Mih. Technik, 1014).—Pieces of very fresh tissue of not more than half a centimetre in thickness are put for at least four days into 10 per cent, formol. They are then mordanted for four or five days in an incubating stove (or for at least eight days at the temperature of the laboratory) in a solution containing 5 per cent, of neutral acetate of copper, 5 per cent, of acetic acid, and 2^ per cent, of chrome alum, in water. Add the alum to the water, raise to boiling pomt, and add the acetic acid and the acetate, powdered (or [Encycl, p. 1019]](https://iiif.wellcomecollection.org/image/b21462586_0466.jp2/full/800%2C/0/default.jpg)
