The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1905
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.
480/572 page 466
![operation of depigmentation may be combined with that of staining ; if you stain with borax-carmine and wash out in the last-mentioned liquor, the pigment will be found to be removed quicker than the stain is washed out. The operation may be carried out on sections, but it is better to use portions of retina of 2 to 5 mm. in thickness. Grenacher mounted his preparation in castor oil, see § 462. Similar methods are recommended by the same author for the eyes of Heteropoda (see Ahh. naturf. Gen. Halle-a.-S., 1886; Zeit. iviss. Mih., 1886, p. 248). Lenhossbk {Zeit. wiss. Zool., Iviii, 1894, p. 636 ; Arch. mik. Anat., xlvii, 1896, p. 45) applies the method of GtOLGI to the eyes of Oephalopods. Similarly Kopsch {Anat. Anz., xi, 1895, p. 362), but using formol instead of the osmic acid. Hesse {Zeit. wiss. Zool., Ixviii, 1900, p. 418) fixes eyes of Heteropoda with 1 of formol to 4 of water, and (p. 456) bleaches those of Cephalopoda by the methods of Geenachek .and that of Jander, § 595. 868. Eyes of LamellibrancMata.—See Patten, Mitth. Zool. Stat. Neapel, vi, 1886, p. 733, and Rawitz, Jena, Zeit. Naturiv., xxii, 1888, p. 115, and xxiv, 1890, p. 579 (bleaches witb. caustic soda); see § 596. Hesse {op. cit., last §, p. 380) employs the method of Jandee for Area. He fixes the eye of Peden in 10 per cent, formol for five minutes, followed by sublimate or picro- nitric acid. 869. Shell.—Sections of non-decalcified shell are easily obtained by the usual methods of grinding, or, which is often a better plan, by the methods of v. Koch or Ehrenbaum, §§ 190, 191. Moseley {Quart. Journ. Mic. Sci. [2], xxv, 1885, p. 40) decalcifies with nitric acid of 3 to 4 per cent, and then makes sections. This method serves for the study of the eyes of CHiTONiD^i;. 870. Injection of Acephala (Flemming, Arch. onih. Anat., 1878, p. 252).—To kill the animals freeze them in a salt-and- ice mixture, and throw them for half an hour into lukewarm water. They will be found dead, and in a fit state for in- jection. The injection-pipe may now be tied in the heart,](https://iiif.wellcomecollection.org/image/b21462586_0480.jp2/full/800%2C/0/default.jpg)
