The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1905
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.
482/572 page 468
![Arthroiwda. 872. General Methods for Arthropoda.—As general methods for the study of cliitinous structures, the methods worked out by .Paul Mayer (see §§ 8, 101, 251, and 252) are excellent. It is, at all events, absolutely necessary, in the preparation of entire organisms or tmopened organs, that all processes of fixation, washing-, and staining should be done with fluids possessing great penetrating power. Hence picric acid com- binations should in general be used for fixing, and alcoholic fluids for washing and staining. Concentrated picro-sulphuric acid (or picro-nitric) is the most generally useful fixative, and 70 per cent, alcohol is the most useful strength for w-ashing out. Alcoholic picro-sulphuric acid may be in- dicated for fixing in some cases. But if the animals or organs can first be properly opened, the usual methods may be employed. 873. Crustacea.—Some forms are very satisfactoinly fixed with sublimate. Such are the Copepoda and the larvse of Decapoda. It is sometimes indicated to use the sublimate in alcoholic solution. Some Copepoda, however {Copilia, 8apphirina), are better preserved by means of weak osmic acid, and so are the Ostracoda. In many cases the osmic acid wall produce a sufficient differentiation of the tissues, so that further staining may be dispensed ^vith ; so for Copilia and PhyUosoma. The pyrogallic process (§ 383) may often prove helpful in the study of such forms. Gibsbkecht takes for marine Copepods a concentrated solution of picric acid in sea water, to which a little osmic and acetic acid may be added. For fresh-water forms, Zacharias {ZooJ. Anz., xxii, 1899, p. 72) takes chromo-acetic acid. For Ostracoda, Mullek {Fauna u. flora d. Golfes von Neapel, xxi [Ostracoda], 1894, p. 8) recommends fixing in a mixture of five parts of ether and one of absolute alcohol, followed by 70 per cent, alcohol. Wolteeeck [Zeit. iviss. Zool, Ixiv, 1898, p. 601) fixes Gypris with picric acid, saturated, 100 parts, sublimate, saturated, 50 parts, and 2^ to 5 of acetic acid, Hekbst [Arch. Ihitwichmech., ix, 1899, p. 292) finds 1 per cent, formol in sea-water good for Crustacea in general.](https://iiif.wellcomecollection.org/image/b21462586_0482.jp2/full/800%2C/0/default.jpg)
