The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1905
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.
486/572 page 472
![881. Injections (Aracliuida and Crustacea especially).— AiME ScHNJiijjEii [TaUettes Zool., i\, 1892, p. 123) recom- mends lithog-raphic Indian ink, the animals being narcotised with chloroform, then injected and thrown into strong alcohol. Similarly Causaed {Bull. Sc. France Belg.,xxix, 1896, p. 16). 882. Arctiscoida (Doyere, Arch. mile. Anat., 1865, j). 105).—Ex- amination of living aniuaals tifter paiiial asi^liyxiation in boiled water. See early editions. Vermes. 883. Enteropneusta.—Lo Bianco (op. cit., p. 460) fixes with picro-sulphuric acid or 05 per cent, chromic acid, Avith previous narcotisation with alcohol if desired. WiLLEY {Zool. Results, etc., iii, 1899, p. 325) takes 100 parts 1 per cent, chromic acid with 2 of 1 per cent, osmic acid, for 12 hours. 884. Myzostoma.—Wheeler {Mitth. Zool. Stat. Nea.joel, xii, 1896, p. 227) fixes with sublimate or picro-acetic acid. 885. Chaetopoda: Cleansing Intestine.—KiiKENTHAL {Joum. Roy. Mic. Soc, 1888, p. 1044) puts Lumhricus into a tall glass vessel which has been filled up with bits of moistened blotting-paper. They gradually evacuate the earthy j^articles from the gut, and fill it instead with pa]3er. VoGT and Yung {Traite d' Anat. Gomp. Prat., v) recom- mend coffee-grounds instead of paper j paper becomes rather hard when imbedded, whereas coffee-grounds cut fairly well, JoEST {Arch. Enfwickhtngsmech. v., 1897, p. 425) simply keeps the worms for a few days in moist linen, and finds the gut empty. 886. Chaetopoda : Fixation.—Lumhricits may be aneesthetised \)j putting the animals into water Avith a few drops of chloro- form. Perriee j)refers not to let the chlorofoi-m act directly in solution on the animals, but to put them into water in a shallow dish, set up a watch-glass with chloroform in the corner of it, and cover the whole. In half an hour the Avorms will be more or less narcotised, and if allowed to remain A\dll die in a state of extension. Cerpontaine {Arch, de Biol., x, 1890, p, 327) recommends](https://iiif.wellcomecollection.org/image/b21462586_0486.jp2/full/800%2C/0/default.jpg)
