The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1905
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.
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![that very perfect natural injections may often be obtained from leeches that have been hardened in weak chromic acid or other chromic liquid. He considers that these injections are the best for the purpose of the study of the circulatory system by means of sections. Jacquet {Mitth. Zool. Stat. Nea'pel, 1885, p. 298), for artificial injections, puts leeches into water with a very small quantity of chloroform; they soon fall to the bottom of the vessel and remain motionless. They should be allowed to remain a day or tAvo in the water before injecting them. Nervous system.—Impregnation with gold. Beistol {Journ. of Morph., XV, 1898, p. 17) kills in formic acid of 15 to 20 per cent., puts for twenty-five minutes into 1 per cent, gold chloride, reduces in formic acid of 1 per cent, (twelve to eighteen hours), and imbeds in parafiin. See also §§ 350, 377, 380, and 774. . 891. Gephyrea.—Yogt and Yung (Anat. Comp. Prat., p. 373) direct that Siplmnculus nudus be kept for some days in perfectly clean basins of sea water, changed every day, in order that the intestine of the animals may be got free from sand, and then anaesthetised with chlorofoi-m, under which treatment they die extended. Waed [Bull. Mils. Comp. Zool., Gamhridge, Harvard Coll., xxi, 3, p. 144) puts them into a shallow dish with sea water and pours 5 per cent, alcohol in a thin film on to the surface of the water. After four to eight hours, if the animals make no contractions on being stimulated, they may be removed to 50 per cent, alcohol. S. Lo Bianco says killing with 0*5 per cent, chromic acid or with O'l per cent, chloi-al hydrate in sea water may be tried, but either method is uncertain. Phascolosoma and Phoronis should be treated by the alcohol method. Apel {Zeit. wiss.' Zool, xlii, 1885, p. 461) says that Po-iapulus and Ealicryptus can only be satisfactorily killed by heat. The animals may either be put into a vessel with sea water and be heated on a water-bath to 40° C. ; or they may be thrown as ra]ndly as possible into boiling water, which paralyses them so that they can be quickly cut open and thrown into ^ per cent, chromic acid or picro-sulphuric acid.](https://iiif.wellcomecollection.org/image/b21462586_0489.jp2/full/800%2C/0/default.jpg)
