The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1905
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.
495/572 page 481
![has had good results with the rapid Grolgi method only on Distoma liepaticvm^ and prefers methylen blue. Ha VET [La Cellule, xvii, 1900, p. 353) has also had results with the Golgi method on this form, and also with thionin, (after fixing with sublimate), which demonstrates tigroid substance. CercariiB.—Schwaeze {Zeit. wiss. ZooL, xliii, 1886, p. 45) found that the only fixing agent that would preserve the histological detail of these forms was cold saturated sublimate solution warmed to 35°—40^ C. For an indifferent liquid, Hopmann {Zool. Jalirh., xii, 1899, p. 176) takes 1 part of white of egg in 9 of normal salt solution. 898. Turbellaria.—For Bhahdoccela, Beaun {Zeit. iviss. Mik., iii, 1886, p. 398) proceeds as follows : For preparing entire animals, the specimens are got on to a slide, lightly flattened out with a cover, and killed by running under the cover a mixture of three parts of liquid of Lang with one of 1 per cent, osmic acid solution. Other fixing media than that described were not satisfactory. (Bohmig [ibid.'], commenting on this, says that for some of the tissues, such as muscle and body parenchyma, nitric acid and picro-sulphuric acid are very useful.) Sections may be made by the paraffin method. Delage {Arch, de Zool. exp., iv, 2, 1886) recommends fixation (of Rhabdocoela Acoela) by an osmium-carmine mixture, for which see loc. cit, or by concentrated sohition of sulphate of iron. Liquid of Lang was- not successful. For staining, he recommends either the osmium-carmine or impreg- nation with gold (i formic acid, two minutes ; 1 per cent, gold chloride, ten minutes ; 2 per cent, formic acid, two or three days in the dark. It is well to allow an excessive reduction to take place, and then Ughteu the stain by means of 1 per cent, solution of cyanide of potassium). Bohmig {Zeit. wiss. Mih., iii, 1886, p. 239) says that he has obtained veiy mstructive images with Plagiostomidse fixed with sublimate ajid stained with the osmium-carmine. Geapp {TurheUaria Acoela, Leipzig, 1891; Zeit. iviss. Mile, IX, 1892, p. 76) says that chromo-aceto-osmic acid, followed by hiematoxylin, is good for the skin, but will not afford a satisfactory preservation of the Rhabdites, which in Acoela 31](https://iiif.wellcomecollection.org/image/b21462586_0495.jp2/full/800%2C/0/default.jpg)
