The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1905
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.
500/572 page 486
![young- 8ynafipp formed from Auricularia. Without this ])rocaution you generally get preparations of larvas either shut up {Peittacriiiitti), or entirely deformed by contraction (young Syna2'>tv(i). See also MacBride on the development of Amphiura squamata. Quart. Journ. Mic. Sci.,xxxiv, 1892, p. 131 (osmic acid followed by liquid of Miiller and alcohol; decalcification with nitric acid in alcohol; stain- ing with Mayer's paracarmine or hsemalnm); and Seeligee on the de- velopment of Antedon, Zool. Jahrh., Abth. Anat., vi, 1892, p. 161. MacBride {Quart. Journ. Mic. Sci., xxxvili, 1896, p. 340) fixes larvai of Asterina in osmic acid, brings into liquid of Miiller for twelve to four- teen hom-s, imbeds in celloidin followed by paraffin (see § 184), and stains sections with carmahim or Delafield's hasmatoxylin, best after a foregoing stain of twenty-four boiirs in borax carmine. Goelenteo^ata. 904. Thread-cells.—Iwanzoff (Bull. Soc. Nat. Moscou, x,1896, p. 97) advises for tlie Nematocysts of Actiniae maceration by the Hert- wiGs' method, § 548, or better, fixation for two to five minutes with vapour of osmium followed by a sbort washing with sea water or distilled water. For Medusae he also advises the Hertwigs' method, § 543, or treat-' ment with a solution containing methyl green and gentian violet with a little osmic acid. Little {Journ. App. Mic, yi, 190d, p. 2116; Journ. Roy. Mic. Sac, 1903, p. 237) kills Hydra in hot saturated sublimate in 70 per cent, alcohol, washes with alcohol, stains for five minutes in strong solution of methylen blue, dehydrates rapidly, clears with cedar or bergamot oil, and mounts in balsam. Nematocytes blue, the rest unstained. 905. Actinida.—Narcotisation.—For suitable narcotisation methods see §§ 13 to 28. Fixation.—In Le Attinie, Fauna u. Flora d. Golfes v. Neapel, Andees says that hot corrosive sublimate often gives good results. In the case of the larger forms the solution should be injected into the gastric cavity, and a further quantity of the liquid be poured over the animals. Freezing sometimes gives good results. A vessel contain- ing Actinije is put into a recipient containing an ice-and-salt freezing mixture and surrounded by cotton-wool. After](https://iiif.wellcomecollection.org/image/b21462586_0500.jp2/full/800%2C/0/default.jpg)
