The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1905
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.
504/572 page 490
![CHAPTEJI XXX\'J:. 15 to 20 per cent, solution of the salt is suddenly added to the sea water containing the animals. As soon as they are fixed (which happens in a few minutes) a few drops of nitric acid are to be added and mixed in (this is in order to prevent the formation of precipitates), and the whole is left for four to five hours. The specimens are then to be hardened before bringing them into alcohol. Bedot recommends that this be done with strong solution of Flemming, Avhich should be added to the sohition of sulphate containing the Siphonophore, about two volumes of it being taken for one of the sulphate solution. The whole should be left for at least twenty-four hours. Lastly, a few drops of 25 per cent, alcohol should be added to the fluid with a pipette, being dropped in as far as possible from the colony, which should be disturbed as little as possible; and further alcohol, of gradually increasing strength, should be added so gradually that the strength of 70 per cent, be not attained under fifteen days at least. Ninety per cent, alcohol should be used for definite preserva- tion. I have tested this method, and find that it enables one to preserve specimens with all their swimming-bells and polyps in situ, a result which is not obtained by means of the usual methods. Fkiedlaendee [Biol. Centralbl., :si, 1890, p. 483) inundates the animals with a mixture of 125 parts cupric sulphate, 125 parts zinc sulphate, and 1000 parts water. Lo Bianco (loc. cit., p. 454) employs for the majority of Siphonophora a mixture of 10 c.c. of saturated solution of corrosive sublimate with 100 c.c. of 10 per cent, solution of cop]3er sulphate. This is used as in Bedot's process. Diphyes, Rhizophysa, and Physalia, however, are killed with sublimate solutions; Velella with chromo-picric acid, or a mixture of 100 c.c. of sublimate solution with 50 c.c. of 1 per cent, chromic acid; Poriy'dahj poisoning with liquid of Kleinenberg. KoROTNEiVs method has been given, § 15. I have seen Physophora very successfully killed by the cai-eful adminis- tration of ether. Preservation, after fixation and washing, is greatly sim- plified by the use of formaldehyde instead of alcohol ( Webee) . Daviuopp {Anat. Anz., xi, 1896, p. 505) fixes in formol.](https://iiif.wellcomecollection.org/image/b21462586_0504.jp2/full/800%2C/0/default.jpg)
