The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1905
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.
509/572 page 495
![KoRSCHELT {{hid., Y, 1882, 13. 217) emi^loys in the same way 1 per cent, osmic acid, or, for Amcebge, 2 per cent, chromic acid. Lansberg {ibid., p. 336) advises the same reagents, but recommends bringing the organisms into the fixing liquid with a pipette. For fixation with iodine (Kent) or iodine vapour (Overton) see § 88. For sulphurous acid, § 68. Cattaneo [Bollettino Scientifico, in and iv; Jourii. Boy. Mic. Soc, 1885, p. 538) recommends fixing for a few minutes with per cent, solution of chloride of palladium. Brass {Zeit. iviss. Mik., 1884, ]3. 39) employs a mixture of 1 part each of chromic acid, platinum chloride, and acetic acid with 400 to 1000 parts of water. Certes {Comptes rend., Ixxxviii, 1879, ]). 433) fixes with 2 per cent, osmic acid, or its vapom-s (10 to 30 minutes). For details see previous editions. Du Plessis (Yogt et Tung, Traite, Anat. Covip. Prat, p. 92) recom- mends fixation witli 0-2 per cent, solution of corrosive sublimate. Let the preiaaration di-y up, and if the organisms have preserved their shape, stain and mount in balsam. This seemingly barbarous procediu-e is said to give fine preparations when successful. FoL {Lehrb., p. 102) fixes delicate marine Infusoria {Tin- tinnodea) with the perchloride of iron solution (§ 8-5), added to the water containing them, and stains with gallic acid as directed, § 384. Lo Bianco [loc. cit., p. 444) fixes Gregarin^ with picro-sul- phuric acid (one hour), Vorticellte with hot sublimate, Acinetae with sublimate in sea water, or with osmic acid, Thalassicola with 0-.5 per cent, chromic acid (one hour), Acanthometrse and Aulacanthse with 50 per cent, alcohol or Avith concentrated sublimate, or by adding a little osmic acid to the water. For Sph^rozoa he proceeds as Brandt, § 922. ZoGRAP fixes Rhizopoda and Infusoria as Rotatoria, § 892, but without narcotisation. See also the methods of Fabee-Domergtje, An::., de Microgr., ii, 1889. p. 545, and 1890, p. 50; Sghewiakoff, Biblioth. Zool., v, 1889,'p. 5; Journ. Boy. Mic. Soc, 1889, pp. 832, 833 ; Zoja, Boll. Sci. Pavia,' 1892 • Zeit. wiss. Mik., ix, 1893, p. 485 ; Longhi, Bull. Mas. Zool. Univ. Genovci, 4,1892 ; Zeit. loiss. Mile, ix, 1893, p. 483 ; Lauterborn, Zeit. xoiss. Zool,](https://iiif.wellcomecollection.org/image/b21462586_0509.jp2/full/800%2C/0/default.jpg)
