The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1905
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.
512/572 page 498
![or Zeit. iviss. Mi]c.,xxi, 1905, p. 491, and Giemsa, p. 522, or Gentralh. BaU., 1904, p. 308. 925, Flagellata.—Lauterborn {Zeit. wiss. Zool., lix, 1895, p. 170) fixes Ceratium for about ten minutes in liquid of Fleninaing, puts into alcohol for twenty-four hours, brings back into water, bleaches if necessary with hydrogen peroxide, and stains with picrocarmine or Delafield's hsematoxylin. He also imbeds in paraffin, § 921, and stains sections with iron hsematoxylin, Wasielewski and Senn {Zeit. Eyg., xxxiii, 1900, p. 451) study Heiyetomonas by making dry cover-glass films of blood, fixing them by heat or absolute alcohol, and staining by the method of Romanowsky, § 719, Zachaeias {Zool. Anz., xxii, 1899, p. 72) fixes Uroglena, etc, with a mixture of 2 vols, saturated aqueous solution of boracic acid and 3 of saturated sublimate. 926. Stains for Flagella.—The process of Romanowsky and Zettnow, § 719, will give a red stain of the flagella of some forms. The method of Lopplee has run through several forms {Centralhl. BaUeriol., vi, 1889, p. 209; vii, 1890, p. 625; Zeit. loiss. Mil., vi, 1889, p. 359; vii, 3, 1890, p. 368; Jotirn. Roy. Mic. 8oc., 1889, p. 711; 1890, p. 678), of which that given here is the latest. To 10 c.c. of a 20 per cent, solution of tannin are added 5 c.c. of cold saturated solution of ferrous sulphate and 1 c.c. of (either aqueous or alcoholic) solution of fuchsin, methyl violet, or Wollschwarz.''^ Cover-glass preparations are made and fixed in a flame in the usual way, special care being taken not to over-heat. Whilst still warm the preparation is treated with mordant {i. e. the above-described mixture), and is heated for half a minute, until the liquid begins to vaporise, after which it is washed in distilled water and then in alcohol. It is then * Tlie luixtui-e will reqviire/or some forms the addition of a few di-ojis of 1 per cent, solution of caustic soda; e. g. for typhoid bacilli, 1 c.c.; for Bacillus suhUlis, 28 to 30 drops ; for bacilli of malignant oedema, 36 to 37 drops. Some other forms will require besides the addition of a trace of siUphui-ic acid to the soda solution—so for cholera bacteria, half a di-op to 1 drop ; for Spirillum rubrum, 9 drops.](https://iiif.wellcomecollection.org/image/b21462586_0512.jp2/full/800%2C/0/default.jpg)
