The essentials of practical bacteriology : an elementary laboratory book for students and practitioners / by H. J. Curtis.

  • Curtis, Henry J.
Date:
1900
Catalogue details

Licence: In copyright

Credit: The essentials of practical bacteriology : an elementary laboratory book for students and practitioners / by H. J. Curtis. Source: Wellcome Collection.

Provider: This material has been provided by The University of Leeds Library. The original may be consulted at The University of Leeds Library.

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    METHODS OF TESTING ANTISEPTICS ^^67 endemic when once it has ^^^^^^ J; may point to the a simple tonBilUti. ^^'^T oommuni, i. commonly found *^A variety of Proteus mirabilis is also not uncommonly found the vel-y long Involution forms.-sinuously curved fllan.entB W.th spLIl^'hap 'd swelhngs along thek course, or at the.r extrem.taes.- being characteristic. LESSON XXIII METHODS OF TESTING ANTISEPTICS AND DISINFECTANTS 1. Take four tubes containing 10 c.c. of nutrient broth, a, b, c, cl. (i) Inoculate (a) with a loopful of. ^^g^ q^-^ ^^.^ to be incubated B. 'prodigiosus. I and used as controls, for (ii) Inociilate {h) with a loopful of j comparison with (c) and {d). B. tyfJiosus (iii) Add -iV c.c. of a 5 per cent. ^ Inoculate (c) and {d) with a solution of carbolic acid to (c).l loopful each of B. ]orodi- ■ ^ giosiis and B. tyioJwsiis. Incubate at 37° C. (iv) Add i c.c. of a 5 per cent, solution of carbolic acid to {d). On comparing these tubes, after a few days' incubation, {a) and (&) will show vigorous growths of B. prodigiosits and B. typliosus re- spectively ; in (c), turbidity due to B. typhosus only will be seen; B. 23rodigiosus having succumbed to the antiseptic ; whilst in {d), no growth of either organism will be visible. II. (i) Prepare anthrax silk threads ^ as follows :— Silk threads, one inch long, are sterihsed in the steamer, and dipped for an hour in broth, or normal saline solution, in which has been ' These should be previously prepared for the class.