The large-scale contained use of biological agents / Advisory Committee on Dangerous Pathogens.
- Great Britain. Advisory Committee on Dangerous Pathogens
- Date:
- 1998
Licence: Open Government Licence
Credit: The large-scale contained use of biological agents / Advisory Committee on Dangerous Pathogens. Source: Wellcome Collection.
48/68 (page 40)
![Ohele, else: ‘ey wi fe) o: Yor Jé: Ye, (0) 0: “6: “oll fe, “el fe) (ete) (on el lewie! Ten 4eh 0] 10) 19) 66, je. os 0) e) fe! ‘ejite) 6: 6. te) «161 vel fo! onde so) Jel erm er veda] mejute cauie, me! is! HEPA HEPA HEPA HEPA ++ uP + = Corridor | Workplace Corridor | Workplace GMP area Non-GMP area Containment Level 2 or above Containment Level 2 or above Figure 15 Air pressure requirements for GMP and non-GMP areas 149 In order to balance both the health and safety and GMP requirements of processes using Hazard Group 2 agents, consideration can be given to having a negative pressure cascade system of the order of 30 Pa, 60 Pa and 90 Pa relative to the outside atmosphere. The minimum recommended differential between rooms is 15 Pa (1 Bar = 100 000 Pa). 150 It is necessary under these conditions to have a balanced air flow using dampers and alarms to counteract the effects of normal events such as opening doors, as well as abnormal events such as a room leak or system failure. 151. Alternatively, consideration can be given to the use of a corridor or lobby with a HEPA-filtered positive air flow. The air pressure in the lobby should be positive to both the plant side to prevent or minimise escape of airborne organisms, as required by the Containment Level in question, and also to the outside to prevent or minimise contamination. 152 In sucha system the corridor would be at positive pressure to the processing area, through a cascade, consisting of first the changing lobby, then the containment area and thirdly any contained equipment. The fermenter would be at positive pressure to the contained area, but negative to the outside atmosphere. a) xe: “a Miat eters 6 (ee! 6 fie! tee le. 8: 1, ee) “aie, Js:\/e! e160 1o1 ie! (ee: leo) emia! “0, (eo) ceie Comic ue sis seule) ose! (oMialuaMeiholie leliacs gelis'alts)](https://iiif.wellcomecollection.org/image/b32230163_0048.jp2/full/800%2C/0/default.jpg)