Blood diseases in clinical practice / by P. Morawitz ; translated from the second German edition by A. Piney.

  • Paul Morawitz
Date:
1933
Catalogue details

Licence: In copyright

Credit: Blood diseases in clinical practice / by P. Morawitz ; translated from the second German edition by A. Piney. Source: Wellcome Collection.

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    are useful to the practitioner. I particularly recommend the eosin-methylene blue of Jenner-May-Gmnwald. More complete, indeed almost perfect, pictures can be obtained by Pappenheim’s method of staining first with Jenner and then with Giemsa. Jenner’s method is especially convenient in practice, because no preliminary fixations of the films is neces¬ sary. The stain (obtainable from Grübler, of Leipzig x) is dissolved in methyl alcohol, which acts as a fixative during the staining process. Air-dried films are placed in the solution for three to five minutes, rapidly swilled with distilled water, and dried with filter paper. The film is then ready for examination with the oil-immersion lens. If one wishes to use Giemsa’s solution, the Jenner- stained film is swilled with distilled water and immedi¬ ately placed in diluted Giemsa solution (2 drops of staining solution to each 1 c.c. of distilled water) for about twenty minutes. Nuclear structure and parasites, which are often rather pale after Jenner, are beautifully shown. On the whole, Jenner’s stain give well-coloured, instructive preparations. The erythrocytes are red, and all nuclei are blue. The various leucocyte granules are well stained : the large granules of the eosinophiles are brilliant red ; the finer ones of the neutrophiles are reddish-violet ; and the coarse granules of the basophiles are blue-violet. Stained films can give much exact information about the exact proportions of the different leucocytes, which is impossible in wet films. If the fresh film has shown an increase of leucocytes, the stained one will demon¬ strate which variety of white cell is responsible for the leucocytosis. If practically only lymphocytes are seen, 1 In this country from any good firm dealing in microscope dyes (Tr.).
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