Royal Society of Medicine, London

Date:
1971
Reference:
PP/CRI/E/1/19/13
Catalogue details

Licence: In copyright

Credit: Royal Society of Medicine, London. Source: Wellcome Collection.

    15/118
    Cellular selection regulating variations in affinity during the antibody response. T ^ p i^iijispt.of ^ lot BioJ Karolinsks Institutet,IO4OI Stockholm,Sweden. Cellular events in the B-ccll line of immune corape tsnt cells have been studied.Antibody affinity, a marker on the variable region of the iinmuncglobu-b lins c ^ì body plaque-forming cells (PPC:s) to inhibition by free antigen »¿s used at- a measure of affinit,7.Thus,affinity of antibodies could, be studied at the cellular lev el. The cellular selection was recorded during different experir::—>■ tal conditions: where the effective antigen concentration was varied : differenti doses of ant i gen ,-varioue times after immunisa ti. on, administration of passive' 7 ] antibody »induction of partial to le rane e, if fini ty 'as found, to be a property ! regulated within the £~cell line,'since different modifications of the immune 3 r_ -nei o r-^ : r 5 ' r j- , , , in the affinity of the anti-hapten response (B-ccll function)«Cell surface located; antigen specific receptors were found to express a similar affini:,.- j for the antigen as the immunoglobulin released by the same cell,since it was possible to fractionate immunocompetent cells with regard to affinity on anil- go;; coated iinmunoadsorbent columns«A clonal distribution of immunological me mory ceils with regard to affinity was shown by limiting dilution assay.The isolated clones showed a marked homogeneity with regard to affinity.
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