Annual report on the Medical Research Institute / 1909-.

  • Nigeria. Medical Research Institute.
Date:
[1915]
Catalogue details

Licence: Public Domain Mark

Credit: Annual report on the Medical Research Institute / 1909-. Source: Wellcome Collection.

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    172. After 20 hours only spherical forms were seen, many of them actively amoeboid. At the end of 24 hours, 26, 28 and 30 hours, the only change noted was a steady increase in size, although the rate of growth was not equally rapid in all, and in no instance was more than about a quarter or the red cell occupied by the parasite. 173. At the end of 32 hours it was obvious that there were two forms of the protozoon, one larger and now with fine discrete grains of black pig¬ ment, occupying very nearly half the host cell, and one smaller, occupying only about a quarter of the erythrocyte and not showing any pigment. 174. After 34 hours’ incubation there was still the difference in bulk between the two forms of the parasite, but all then contained pigment. 175. After 44 hours increase in size both of cytiplasm and chromatin was noted, and the same condition ruled at the end of 54 hours. 176. When the 56th hour was reached, however, the distinction between the two forms was very evident, only a few parasites being in the pre-rosette stage, and the great majority obviously showing the characters of the sexual generation. 177. Four hours later (60 hours in all) the blood picture was particularly striking. Auto-agglutination was well marked in the fresh smear. In the stained smear most of the parasites were gametocytes. Some rosettes were observed, ingested by the large mononuclear cells as well as by the neutrophil polymorphs, and a certain number of rosettes also showed signs of degenera¬ tion. Apparently, however, some rosettes had sporulated in the interval between the 56th and the 60th hour, as some tiny rings were seen and red cells doubly or trebly infected by these were noted. In addition, there was a fair proportion of somewhat compact and spherical parasites retaining the appearances of those forms noted as in the majority in the smears taken at the 56th hour. 178. After 62 hours, agglutination of the gametocytes was a marked feature of the fresh smears. There were very few definitely recognisable schizonts, and these appeared to be degenerated. A few of the more compact spheres were still observable; these did not suggest that they had developed from the small ring forms noted at the 60th hour, but rather gave the impression that most of them were the spherical compact forms which had not progressed. A few, however, showed abundant chromatin. 179. At the end of 66 hours, gametes predominated, agglutination of these was well marked, and the compact spherical forms were few. Only a very few forms were seen, evidently of the second generation noted at the 60th hour. 180. After 68 hours, gametes still bulked largely in the stained films, but they all showed signs of degeneration. The forms already alluded to as probably proceeding from the second generation of schizonts were very few, but showed increase in bulk. 181. At the end of 78 hours all forms of the parasite had very appre¬ ciably lessened in number, but such as remained consisted of a few gametes and a few forms in the pre-rosette stage. 182. After 80 hours a few rosettes were observed, most of them infected by the leucocytes, but a few were free and some of these had shed their spores. 183. Four hours later, 84 hours in all, practically all the parasites showed signs of degeneration, a few ingested rosettes were still observable and some tiny spheres were seen inside the red cells, but the gametes had practically disappeared.
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