The blood : how to examine and diagnose its diseases / by Alfred C. Coles.
- Coles, Alfred C. (Alfred Charles)
- Date:
- 1905
Licence: In copyright
Credit: The blood : how to examine and diagnose its diseases / by Alfred C. Coles. Source: Wellcome Collection.
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![good as that recommended by Muir or Gulland. Absolute alcohol alone may be used instead. Formol promises to be very useful, and as its action is rapid, most convenient for clinical examinations. [Formol is a 40% solution of formic aldehyde in water.] Benario, for fixing blood preparations, uses a 10% watery solution of formol, and of this stock solution he dilutes one part with ten of alcohol when it is required for use. The preparations remain in it for one minute, and can then be stained without drying. The mixture—formol one part, water nine, alcohol ninety parts—could be used if well stoppered to prevent evaporation. For detailed study of nuclear structure, and for the demonstration of karyokinesis, fixation by Flemming’s solution or by Muir’s method may be used. I find the following very satisfactory:— Mullers Method.—The films are heated, and when cold placed in a saturated aqueous solution of picric acid for twenty-four hours. They are then washed for several (twelve to twenty-four) hours, to remove as much as possible of the acid, and subsequently stained for several hours in very diluted Bohmer’s hasmatoxylin solution. They are finally washed, and any overstaining with hEematoxylin removed by weak hydrochloric acid alcohol, under frequent examination with the microscope. They should be cleared with xylol or cedar oil, and mounted in Canada balsam. I have obtained very fine specimens by this means. They may, however, be stained for a short time in undiluted borax carmine, instead of hcematoxylin.](https://iiif.wellcomecollection.org/image/b28138855_0048.jp2/full/800%2C/0/default.jpg)
