Studies upon hypersusceptibility and immunity / by M.J. Rosenau and John F. Anderson.

  • Milton Rosenau
Date:
1907
Catalogue details

Licence: In copyright

Credit: Studies upon hypersusceptibility and immunity / by M.J. Rosenau and John F. Anderson. Source: Wellcome Collection.

    14/76 (page 12)
    proflucecl by the tir.st injection come in contact wifhoiU delay; their union is rapid; tlio whole cliarc’c of th(‘, poisonous hubstance is (juickl}' set free and the toxic symptoms are sudden and severe. Besredka and Steinhardt'* had, as a woi'kinj^ liypothesis, the follow- ino” The sensitized guinea pig wliich appears in good health has, in spite of its apparent well-being, perhaps a latent lesion of the brain. A second injection of serum, made into the peritoneal cavity twelve days later, is able to awaken this nervous lesion, resulting in grave s3miptoms or even death. In view of these theoretical considerations it is important to make further studies upon the sensitizing substance in horse serum and other proteid substances. The following experiments show that the filtrate from horse serum after precipitation with ammonium sulphate rendei’s guinea pigs sen- sitive. The filti’ate contains most of the serum albumen and veiy little of the globulins. It is exceedingl}'^ weak in antitoxic strength. G. P. No. 400. Six c. c. antitoxic horse serum (Natl. VIII., 18), intraperitoneally. Marked symptoms. [Previous treatment: 38 days prior, 5 c. c. filtrate of antitoxic serum, precipi- tated (NYBH). Subcutaneously.] This filtrate was kindl}^ furnished us by Dr. W. H. Park from some antidiphtheric serum undergoing the Gibson process of refining. The following experiments show that formaldehyd does not destroy the sensitizing propert}^ of horse serum: G. P. No. 390W. Six c. c. normal horse (roan) serum, intraperitoneally. Severe symptoms. [Previous treatment: 47 days prior, 6 c. c. antitoxic horse serum (Natl. XVIII)+ 1 per cent formalin, 23 hours exposure. Subcutaneously.] G. P. No. 500W. Six c. c. normal horse (roan) serum, intraperitoneally. Very severe s}TOptoms. [Previous treatment: 29 days prior, 3 c. c. normal horse (roan) serum-1-5 per cent formalin, 4 hours 30 minutes exposuure. Subcutaneously.] The results with formaldehyd have a special significance in view of the fact that this active reducing agent is capable of destroying the poisonous properties of tetanus and diphtheria toxines. We have shown before that the sensitizing and poisonous principles in horse serum are not dial^^zable through parchment paper. I rom the following limited experiments it would seem that the sensitizing principle is not dialyzable through a collodion sac when placed in the peritoneal cavity of the animal. G. P. No. B. Six c. c. normal horse (No. 15) serum, subcutaneously. No sj-mptoms. [Previous treatment: 32 days prior, collodion sac containing about 3 c. c. normal horse (No. 15) serum placed in peritoneal cavity.] «Be.>^redka, A., and Steinhardt, Edna: De I’anaphylaxie et de I’anti-anaphylaxie vis-a-vis du st^rum de cheval, Ann. de I’lnst. Pasteur, Vol. 21, No. 2, Feb. 25, 1907, pp. 117-127,