Manual of bacteriology / by Robert Muir and James Ritchie.

  • Robert Muir
Date:
1899
Catalogue details

Licence: Public Domain Mark

Credit: Manual of bacteriology / by Robert Muir and James Ritchie. Source: Wellcome Collection.

Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.

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    sequently shed. Carry carefully back to the laboratory without shaking, and place for twenty-four hours in a cool place, preferably an ice chest. The clear serum will separate from the clotted blood. With a sterile 10 c.c. pipette, transfer this quantity of serum to each of a series of test-tubes which must previously have been sterilised by dry heat. The serum may, with all precautions, have been contaminated during the manipulations, and must be sterilised. As it will coagulate if heated above 68° C, advantage must be taken of the intermittent process of sterilisation at 57° C. « (method B (4)). It is therefore kept for one hour at this temperature ^^^afl X tion is probably an initial stage of coagulation, and is effected by keeping the serum at 650 C. till it stiffens. This temperature is just below the coagulation point of the serum. The more slowly the operation is performed the clearer will be the serum. The apparatus used is seen in Fig. 8. It consists of a rectangular, shallow, covered, hot-water jacket, which can be rapidly heated by an S-shaped Bunsen con- * taining many lateral perforations, from each of which a '■ flame issues. The apparatus rests on four legs, the front two of which can be shortened, and thus the whole tilted ; forward. Tubes containing a suitable quantity of serum ' can thus be laid on their sides without the contents reach- ing as high as the plug. The serum tubes being thus on each of eight succes- sive days. It is always well to incubate it for a day at 370 C. before use, to see that the result is successful. After steri- lisation it is inspis- sated, by which process a clear solid medium is obtained. Inspissa-