Report on the value of sodium silicofluoride as an antiseptic / by T. Jessopp Bokenham.
- Bokenham, T. Jessopp.
- Date:
- [1890]
Licence: Public Domain Mark
Credit: Report on the value of sodium silicofluoride as an antiseptic / by T. Jessopp Bokenham. Source: Wellcome Collection.
Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.
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![who lound that a solution of mercuric chloride administered to an animal would protect it from the effects of a subsequent inocula- tion with anthrax. Before, however, undertaking this Dr. Brunton auggested that it would be well to first test the action of the drutr on fermentation. This I proceeded to do. I took four flasks, and filled them as follows: Flask 1 contained 8 oz. glucose solution in water, sp. gr. 1040 at 15° C.; Flask 2 contained 8 oz. glucose solution in Na, Si F„ soln. (1 to 150), sp. gr. 1040 at 15° C? Flask contained 8 oz. glucose solution in Na^ Si F„ soln. (1 to 300), sp gr. 1040 at 15 C.; Flask 4 contained 8 oz. glucose solution in Na Si F„ soln. (1 to 600), sp. gr. 1040 at 15° C. To each I added a defi- nite quantity of fresh yeast, and then plugged the necks, placing the flasks in an incubator at temperature 25° C. The specific gravities were taken every twenty-fours during seven days. It was thus found that the solution in Flask 1 became of lower specific gravity, whilst that in the others remained unchanged. In experimenting with weaker solutions I adopted another method. A number of tubes of equal length and calibre were taken. These were filled with solutions of Na^ Si Fg of various strengths, each containing besides equal weights of glucose. Some actively growing yeast was then added; the tubes were inverted over pure mercury and placed in an incubator. The volume of CO2, formed by the fermentation could thus be observed from day to day. The result is shown in the accompanying table :— From these experiments it will be seen that a solution of Naj Si Pg (1 to 750) will entirely prevent alcoholic fermentation ; that solutions (1 to 900 and 1 to 1,050) will delay fermentation for a time, but that these latter cannot be considered truly antiseptic, as the growth of the yeast was only delayed. [Experiments just lately performed with nutrient gelatine con- taining Nag Si Fg (1 to 3,000 and 1 to 2,000) have shown that this proportion is quite without effect on the growth of the bacillus anthracis, and that there is no diminution in the virulence 'of bacilli cultivated through several generations on this pabulum.] I next proceeded to try the effect of prolonged administration of the drug to guinea pigs. ExpEBiMENT I.—A saturated tepid solution of NajSiFg was made in saline solution, 75 per cent., and of this 4 cubic centi- metres were injected into the peritoneal cavity of a guinea pig (weight 1 lb. 14 oz.). A short time after the injection it was found with abdomen rigid and legs thrown out; temperature 38° C. in rectum. In spite of all efforts to recover the animal, it became collapsed, with temperature 26.5° C, and abdomen intensely tender. I then killed the animal, and found marked signs of peri- tonitis. No injury could be found attributable to the puncture of the syringe. Experiment II.—Guinea pig (weight 10 oz.) 0.033 grammes, in warm satuiated solution, were injected into the stomach with an oesophageal tube. Animal evinced uneasiness soon after injection; its abdomen became much distended, and it died after fifty-five minutes. Post-mortem appearances as follows: Stomach much dilated, with vessels injected; no signs of injury by the tube; full of pul])y food, with much thick mucus and gas. Intestines con- tained similar mucus. Heart arrested in diastole. Other organs normal. Experiment III.—Guinea pig (female). On April 24th, in- jected 0.02 grammes in solution (1 to 300) into stomach. In three hours repeated the dose. April 25th. Animal quite well and lively. Injected during the day 0.1 gramme of the salt. April 26th. Again injected 0.1 gramme. April 27th. Animal quiet but feeds well. Injected 0.05 gramme in a single dose. Three hours](https://iiif.wellcomecollection.org/image/b22302335_0004.jp2/full/800%2C/0/default.jpg)
