A manual of bacteriology / by A.B. Griffiths.

  • Arthur Bower Griffiths
Date:
1893
Catalogue details

Licence: Public Domain Mark

Credit: A manual of bacteriology / by A.B. Griffiths. Source: Wellcome Collection.

    90/392 page 66
    a sterilised glass ring (f in. diam. x ^ in. high) to a microscopic slide, which has been thoroughly cleaned and sterilised (Fig. 25). The upper edge of the ring is moistened with olive oil or vaseline ; and the cell is covered over by means of a thin cover-glass, previously sterilised by passing it through a Bunsen flame. The surface of the sterilised cover-glass (A) contains a drop of bouillon or other medium, along with the microbes for examination. A drop or two of sterilised water should be deposited at the bottom of the cell; i.e. upon the upper surface of the glass slide. This arrangement forms a miniature damp chamber, in which the growth of microbes may be watched even under the highest powers. After the examination of the cell and its contents, it may be placed in an incubator until it is required again for microscopical examination. To study the action of heat on drop cultures, the warm stages of Schiifer, Eauvier, Israel, Schultze, Strieker, etc., are often used upon the fixed stage of the microscope.-*- The action of various gases on drop-cultures may be watched by a modification of the glass cell as represented in Fig. 25. The gases enter through B. The author has used this device during his researches on the action of certain gases on Bacillus tuhercidosis. The action of the voltaic current or discharges of faradaic electricity may be observed by simple modifications of the drop-culture cell. ' Au excellent piece of glass apparatus is used by the Rev. W. H. Dallinger, F.R.S., for ascertaining the thermal death point of microbes. (See Proc. Boy. Soc, 1878.)
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