Different species of trypanosomata observed in bovines in India / by A. Lingard.

  • Alfred Lingard
Date:
1907
Catalogue details

Licence: In copyright

Credit: Different species of trypanosomata observed in bovines in India / by A. Lingard. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    A secondary reaction eonnnenceJ on the IGtli July wlien an evening temperature of 40’3° C. was registered, and tliis was recorded for several days. The trypanosoma was discovered on the lOtli. In this case also microscopical examination of tlie hlotxl was continued for a considerable peiiod, but no moi-e trypanosomata were discovered, although a daily search was made for them. The T. Ilimalayanum assumes considerable proportions, and although in fresh specimens some difficulty may be experienced in coming to a decision as to the species, never- theless in stained specimens, coloured after any of the modi- hcations of the Romanowsky method, good results may be obtained and little difficulty encountered in coming to a coiTect diagnosis. The ble])haroplast, nutritive nucleus, n^argin of tlie undulating membrane and free tlagellum, stain a deep red, tlie two latter ])ortions of the parasite being particularly well marked, while the endoplasm takes a blue colour. It frequently happens that the body protoplasm is studded with micro- granules, somewhat coarse in structure. These may stain any tint from a dark-red to a deep violet. The posterior extremity (if the organisiit is long and gradually tapers to a point, the blepliaroplast of medium size, nearly circular in form, lies close to one edge of the organism, occupying one- third to one-fourth of its lireadtli, and at a distance from the posterior extremity, which varies between G'5G to 10'GG/^ in different specimens. The nutritive nucleus, as a rule, stains a lighter shade of red than any other portion (.>f the parasite which assumes the same C(,dour. Its size, in single forms of flagellates, varies almost as mucli as its situation, and in many instances is ovate in form, the wider extremity being in proximity with the undulating membrane. The distance observed between the smaller and larger nuclei, in the great majority of organisms, varies from 5’74 to 13T2ju, but a minority exhibit the two nuclei in close apposition, the larger havino’ emigrated to the situation of the smaller one. The undulating membrane in uiature organisms frequently presents an a]:)pearance of having Ijeen artificially gauffered, and the free flagellum in a large majority of ]>arasites in the peripheral blood attains to exactly the same length, namely.