Practical pathology : a manual for students and practitioners / by G. Sims Woodhead.

  • Woodhead, German Sims, Sir, 1855-1921.
Date:
1892
Catalogue details

Licence: Public Domain Mark

Credit: Practical pathology : a manual for students and practitioners / by G. Sims Woodhead. Source: Wellcome Collection.

Provider: This material has been provided by The University of Leeds Library. The original may be consulted at The University of Leeds Library.

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    dissolved in alcohol, to prevent the formation of fungi. Sections hardened in alcohol take up this staining very readily; but when chromic acid, picric acid, or bichromate solution has been used, it is necessary to wash out the whole of the salt, before attempting to stain with this reagent. To stain sections, filter about half a drachm of (a) or ten drops of ip) [to add half a drachm of distilled water] into a watch- glass ; allow the sections to remain for about three or four minutes in {a), or half a minute in (b); wash well in water, and mount in Canada balsam (§ loo, p. 72). Note.—Never put more than two or three sections at a time into the watch-glass, or they cling together, and are unequally stained. Should the staining be too intense, place the sections in a watch-glass, pour a few drops of strong acetic acid over them, then wash and mount. 75. Carmine siaining fluid is es]jecially useful for sections of the central nervous system, and for tissues in which are considerable quantities of fibrous tissue. As a staining reagent for most tissues, it has been superseded by logwood and picro-carmine. To prepare it, take of Pure carmine i drachm. Strong ammonia . . . . i drachm. Water 6 ounces. Triturate the carmine in a mortar, add sufticient water to form a paste, and then add the ammonia, when the paste will at once turn from a bright red to an almost black colour if the carmine is pure. Add the rest of the water and keep the solution in a glass- stoppered botde, with a piece of camphor suspended in the fluid. After carefully washing out picric acid or any of the chromates, a section may be stained rapidly by laying it out on the glass slide, (§ 38, p. 33)j ^rid running a drop or two of the solution over it; allow it to stand for about three to five minutes, and tlien wash in water, not allowing it to remain for more than a couple of seconds, but rapidly transferring it to acidulated water (eight drops of acetic acid to a basinful of water). This last part of the operation must never be neglected, asthe carmine is held in solution by an alkaline fluid,
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