Transfected glioblastoma cells: reduced endocytosis

  • Alex Gray
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Human glioblastoma cells in culture viewed both with phase contrast and fluorescence imaging. The green cell has been transfected with a mutated P10 gene. This produces a catalytically inactive P10 protein which reduces the cell's ability to take up particles by endocytosis. Nuclei are stained blue with DAPI and the transfected cell is full of Green Fluorescent Protein. The red spots are 0.02 micron fluorescent spheres that have been taken up by the untransfected cells much more than by the transfected one.

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