The proteids of serum / by W.D. Halliburton.

  • William Dobinson Halliburton
Date:
[1884?]
Catalogue details

Licence: Public Domain Mark

Credit: The proteids of serum / by W.D. Halliburton. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

    18/48 (page 166)
    This was at first an increase in opalescence: but after keeping the solution at the temperature of 78“ C. for 5-10 minutes, it became a flocculent precipitate. d. 5 c.c. of solution. 3 drops of alkaline solution added ; thus alkalinity — 6 drops. Opalescent 73“ C. Precipitate 80“ C. This also took a long time to form, and then was gelatinous in nature and diflScult to filter. In solutions with more than this amount of alkali added the formation of a precipitate occurred at still higher points; the precipitation of proteid was incomplete; a certain amount of alkali-albumin formed, which remained in solution. Moreover, on adding a larger amount of alkali, a precipitation of magnesia occurred which interfered with the observation. 2. The same solution of serum globulin was used :— a. 5 c.c. of solution. 3 drops of acid solution added : liquid exactly neutral. Opalescence 67“-8“ C. Precipitate 7 6 *5“ C. This was filtered off: the filtrate found to be alkaline : alkalinity of 5 cubic centimetres = 5 drops. h. 5 c.c. of solution. 4 drops of acid solution added; acidity = 1 drop. Opalescence 69“-70“ C. Precipitate 7 5'5 C. c. 5 c.c. of solution. Opalescence. Precipitate. 69-70“ C. 75“ C. 68“-9“ C. 74“ C. 68“-9“ C. 72-5“ C. 68“-9“ C. 70“ C. In all these cases precipitation of the proteid was complete. d. The lowering of the coagulation point after this proceeded more rapidly. Mere addition of the acid at the temperature of the air (27“ C.) rendered the liquid more opalescent. 5 c.c. were taken in each case. Opalescence increased. Precipitate. Acidity = 6 drops. 60“ C. 67“ C. = 7 drops. 60“ C. 64“-5“ C. = 8 drops. 55“ C. 60“ C. The precipitation in all cases was complete. The filtrate after the coagu- ]um had been filtered off was in the first of these three cases neutral, in the two latter it was faintly acid. Acidity = 2 drops. = 3 drops. = 4 drops. = 5 drops.