Advances in gene technology : molecular biology of the immune system proceedings of the Seventeenth Miami Winter Symposium, Miami, Florida, U.S.A., February 11-15, 1985 / edited by J. Wayne Streilein [and others].

  • Miami Winter Symposium
Date:
1985
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Licence: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

Credit: Advances in gene technology : molecular biology of the immune system proceedings of the Seventeenth Miami Winter Symposium, Miami, Florida, U.S.A., February 11-15, 1985 / edited by J. Wayne Streilein [and others]. Source: Wellcome Collection.

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    354 results suggest that the difference in steady-state level of mature 6-niRNA cannot totally account for the low expression of IgD on the surface of neonatal cells. By examining the kinetics of biosynthetic labeling of б chains, one can also compare the translational rate of the ômRNA in adult and neonatal В Ijnnphocytes (3). The kinetics of label incorporation into newly synthesized, intracellular 6 chains (59kd) in neonatal В cells suggest that they are turned over more rapidly than that in adult cells. (Fig. 2A) The rate of accumulation of label into the mature membrane form (66 to 72 kd) also appears to be approximately 50 percent lower in neonatal В cells (Fig. 2B) . These results suggest that the intracellular processing of б chains in neonates is not as efficient as that in adult В cells and may account for the lower cell surface expression of IgD in the latter. We have shown previously that posttranslational modifications may play a role in determining the relative amount of IgM vs. IgD finally expressed in adult В Ijmiphocytes (3). It appears from these studies that posttranslational regulation is also important in determining IgD membrane expression in neonatal В cells. Fig. 2. Relative rate of б polypeptide chain synthesis in neonatal and adult В lymphocytes. T depleted spleen cells from adult or anti-Ig panned (I) spleen cells from 7-12 day neonatal mice were labeled with [^^S] methionine. Aliquots were removed at the indicated times, immunoprecipitated with rabbit anti-6 and analysed by SDS-PAGE as described (3) . The bands corresponding to intracellular б (59kd) and membrane associated б chains (66-72kd) were excised and counted in scintillation cocktail. Two independent experiments from each cell source are depicted. REFERENCES 1. Yuan, D. and P.W. Tucker. J. Exp. Med. 160:564, 1984. 2. Abney, E.R., M.D. Cooper, J.F. Kearney, A.R. Lawton and R.M.Z. Parkhouse. J. Immunol. 120:2041, 1978. 3. Yuan, D., J. Immunol. 132:1566, 1984.
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